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Peptide-affinity Purified Polyclonal Antibody to Annexin A10/Annexin 14 |
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| Catalog No: |
IMG-3169 |
| Contents: |
0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Isotype: |
Goat Ig |
| Clone: |
N/A |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Human, Mouse |
| Predicted React: |
Mouse, Rat |
| Host: |
Goat |
| Application: |
IHC (paraffin): 3-5µg/ml |
| Storage: |
Store at -20°C. |
Recommended
Positive Control: |
Human Pancreas |
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Background
Principal Names – ANXA10; annexin A10; ANX14; annexin 14 Official Gene Symbol - ANXA10 GenBank Accession Number – NP_009124.2 LocusLink ID - 11199 (human) Gene Ontology Terms - calcium ion binding activity |
Antigen
Peptide with sequence FCGDYVQGTIFPAPN, from N Terminus of the protein sequence according to NP_009124.2. |
Application Notes
Preliminary western blot experiments (at 1 ug/ml) gave an ~45-48 kDa band in HeLa, A431 and human kidney lysates. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of ~37 kDa according to NP_009124. Immunohistochemistry: In paraffin embedded Human Pancreas shows staining of islet of Langerhans . Recommended concentration, 3-5µg/ml. |
Genebank Info (Protein)
NP_009124.2 |
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IHC staining of Annexin A10/Annexin 14 in paraffin embedded Human Pancreas using IMG-3169 at 3.8µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
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Reference
1. Morgan RO, Jenkins NA, Gilbert DJ, Copeland NG, Balsara BR, Testa JR, Fernandez MP. Novel human and mouse annexin A10 are linked to the genome duplications during early chordate evolution. Genomics. 1999 Aug 15;60(1):40-9. PMID: 10458909
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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