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Peptide-affinity Purified Polyclonal Antibody to ARPC3 |
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| Catalog No: |
IMG-3180 |
| Contents: |
0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Isotype: |
Goat Ig |
| Clone: |
N/A |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Human, Mouse |
| Predicted React: |
Rat |
| Host: |
Goat |
| Application: |
ELISA: >1:16000
Western blot analysis: 1-3 ug/ml |
| Storage: |
Store at -20°C. |
Recommended
Positive Control: |
human or mouse brain, or HeLa |
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Background
Principal name: ARPC3; ARC21; p21-Arc; actin related protein 2/3 complex, subunit 3, 21kDa; ARP2/3 protein complex subunit p21, RefSeq number: NP_005710 LocusLink number(s): 10094 (human); 56378 (mouse), structural constituent of cytoskeleton; cell motility; Arp2/3 protein complex; actin cytoskeleton. Please note there are two hypothetical proteins, similar to the Arp2/3 protein complex subunit p21-Arc, that are virtually identical (CAC14083, XP_208062). |
Antigen
Peptide with sequence RQFMNKSLSGPGQ, from C Terminus of the protein sequence according to NP_005710. |
Application Notes
Band observed just below 20 kDa marker(predicted MW of 20 kDa according to NP_005710). |
Genebank Info (Protein)
NP_005710 |
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IMG-3180 staining (1 µg/ml) of mouse brain lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
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Reference
1. Welch MD, DePace AH, Verma S, Iwamatsu A, Mitchison TJ.The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly. J Cell Biol. 1997 Jul 28;138(2):375-84. PMID: 9230079
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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