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Monoclonal Antibody to human TLR8 (Toll-like receptor 8)/CD288 |
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| Catalog No: |
IMG-321A |
| Contents: |
0.1 mg in 0.2 ml PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Mouse IgG1, Kappa |
| Clone: |
44C143 |
| Purification: |
Protein G Chromatography |
| Species: |
Human, Mouse |
| Host: |
Mouse |
| Application: |
Flow (Cell Surface): see Cognasse et al. (2005) for details
Flow (Intracellular): 0.5-1 ug/10^6 cells
IHC (paraffin): 5 ug/ml
Western blot analysis: 1-3 ug/ml |
| Storage: |
Store at 4°C, stable for 6 months. For long-term storage, store at -20°C. |
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Background
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). TLR8 gene contains three exons, two of which have coding function (4). TLR8 cDNA codes for a protein of approximate molecular weight of 120 kDa (4,5). |
Antigen
This antibody was developed against a KLH-conjugated synthetic peptide of human TLR8, within amino acids 750-850. It will cross-react with mouse TLR8. |
Application Notes
This antibody tests positive by western blot in transfected cell lysate and human Ramos cell lysate. |
Genebank Info (Protein)
NP_619542 |
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Western blot analysis of TLR8 in human Ramos cell lysate using IMG-321A at 3 ug/ml. IMGENEX’s goat anti-mouse Ig HRP secondary antibody (IMGENEX, 20101) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test. |
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Intracellular flow analysis of TLR8 in 10^6 human lymphocytes using 1 ug of IMG-321A. Shaded histogram represents cells without antibody; green represents isotype control (IMGENEX, 20109); red represents anti-TLR8 antibody. Goat anti-mouse IgG1 PE conjugate (BD) was used as secondary. |
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Immunohistochemical analysis of TLR8 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top) and IMG-321A (bottom) at 5 ug/ml. |
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Reference
1. Muzio M, Natoli G, Saccani S, Levrero M, and Mantovani A. J. Exp. Med. 187: 2097-2101 (1998).
2. Medzhitov R and Janeway CA. Cell 91: 295-298 (1997).
3. Chuang TH and Ulevitch RJ. Biochim. Biophys. Acta 1518 (1-2): 157-161 (2001).
4. Du X, Poltorak A, Wei Y, Beutler B. Eur Cytokine Netw 11(3): 362-71 (2000).
5. Chuang TH and Ulevitch RJ. Eur Cytokine Netw 11(3): 372-378 (2000).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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