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Monoclonal Antibody to TLR8/CD288 (Clone 44C143) Alexa Fluor® 488 Conjugate |
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| Catalog No: |
IMG-321AF488 |
| Contents: |
0.1 mg in 0.2 ml PBS with 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Mouse IgG1, Kappa |
| Clone: |
44C143 |
| Purification: |
Protein G Chromatography |
| Species: |
Human, Mouse |
| Host: |
Mouse |
| Application: |
Flow (Cell Surface): see Xu et al. (2006) for details
Flow (Intracellular): 0.5-2 ug/10^6 cells |
| Storage: |
Product is light- and temperature-sensitive. Store in the dark at 4°C. Do not freeze. |
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Background
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). TLR8 gene contains three exons, two of which have coding function (4). TLR8 cDNA codes for a protein of approximate molecular weight of 120 kDa (4,5). |
Antigen
This antibody was developed against a KLH-conjugated synthetic peptide of human TLR8, within amino acids 750-850. It will cross-react with mouse TLR8. |
Application Notes
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Flow cytometric analyis of CD14-/CD11c+/TLR8+ myeloid dendritic cells (mDCs). Cells were stained for surface markers CD14 (IMGENEX, IMG-6350D) and CD11c, and intracellular stained for TLR8. CD14- cells were gated and stained with AF488-conjugated isotype control (IMGENEX, 20108), and APC-conjugated CD11c (left). CD14- cells were gated and stained with 1 ug AF488-conjugated TLR8 (IMGENEX, IMG-321AF488), and APC-conjugated CD11c (right). |
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Intracellular flow cytometric analysis of TLR8 in 10^6 Ramos cells using 2 ugs of IMG-321AF488. The shaded histogram represents Ramos cells alone, green represents isotype control (IMGENEX, 20108), and red represents TLR8 antibody. |
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Reference
1. Muzio M, Natoli G, Saccani S, Levrero M, and Mantovani A. J. Exp. Med. 187: 2097-2101 (1998).
2. Medzhitov R and Janeway CA. Cell 91: 295-298 (1997).
3. Chuang TH and Ulevitch RJ. Biochim. Biophys. Acta 1518 (1-2): 157-161 (2001).
4. Du X, Poltorak A, Wei Y, Beutler B. Eur Cytokine Netw 11(3): 362-71 (2000).
5. Chuang TH and Ulevitch RJ. Eur Cytokine Netw 11(3): 372-378 (2000).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for six months from the date of purchase.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc. IMGENEX sells Alexa Fluor® dye conjugated antibodies under license from Molecular Probes, Inc. for research use only.
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