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Monoclonal Antibody to human TLR8 (Toll-like receptor 8)/CD288 PE Conjugate |
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| Catalog No: |
IMG-321D |
| Contents: |
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Mouse IgG1, Kappa |
| Clone: |
44C143 |
| Purification: |
Protein G Chromatography |
| Species: |
Human, Mouse |
| Host: |
Mouse |
| Application: |
Flow (Cell Surface): see Xu et al. (2006) for details
Flow (Intracellular): 0.5-2 ug/ 1x10^6 cells |
| Storage: |
Product is light- and temperature-sensitive. Store in the dark at 4°C. Do not freeze. |
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Background
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). TLR8 gene contains three exons, two of which have coding function (4). TLR8 cDNA codes for a protein of approximate molecular weight of 120 kDa (4,5). |
Antigen
This antibody was developed against a KLH-conjugated synthetic peptide of human TLR8, within amino acids 750-850. It will cross-react with mouse TLR8. |
Application Notes
Transfected cell lysate was used as positive control and a protein of approximate molecular weight of 120 kDa was detected in western blotting. Ramos or Raw cells can be used as a positive control in flow cytometry. |
Genebank Info (Protein)
NP_619542 |
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Intracellular flow cytometric analysis of TLR8 in 10^6 human PBMCs using 0.5 ug of IMG-321D, 0.25 ug of anti-human CD14 (IMGENEX, IMG-6350C), and 0.5 ug of isotype control (IMGENEX, 20106). Imgenex's TLR intracellular staining flow assay kit (IMGENEX, 10098K) was used to test this product. |
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Intracellular flow analysis of TLR8 in Ramos cells using 2 ug of IMG-321D. Shaded histogram represents Ramos cells without antibody; green represents isotype control (IMGENEX, 20106); red represents anti-TLR8 antibody. |
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Intracellular flow cytometric analysis of TLR8 in BALB/c mouse splenocytes (lymphocyte gate) using 2 ug of IMG-321D. Green represents isotype control (IMGENEX, 20106); red represents anti-TLR8 antibody. |
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Reference
1. Muzio M, Natoli G, Saccani S, Levrero M, and Mantovani A. J. Exp. Med. 187: 2097-2101 (1998).
2. Medzhitov R and Janeway CA. Cell 91: 295-298 (1997).
3. Chuang TH and Ulevitch RJ. Biochim. Biophys. Acta 1518 (1-2): 157-161 (2001).
4. Du X, Poltorak A, Wei Y, Beutler B. Eur Cytokine Netw 11(3): 362-71 (2000).
5. Chuang TH and Ulevitch RJ. Eur Cytokine Netw 11(3): 372-378 (2000).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for six months from the date of purchase.
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