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Product : Antibody >> Antigen Affinity Purified , Cancer Antibodies , Chromatin , Hormones and Growth Facto.. , Neurobiology >> Androgen Receptor
      
 

Polyclonal Antibody to Androgen Receptor

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-3238
Androgen ReceptorPurified, Peptide Affinity
0.1 mg
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Catalog No: IMG-3238
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 0.1 mg of purified antibody.  0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Isotype: Goat Ig
Clone: N/A
Purification: Antigen Affinity Chromatography
Species: Human
Predicted React: Chimpanzee, Mouse, Rat
Host: Goat
Application: ELISA: >1:64000
Western blot analysis: 0.3-2 ug/ml
Storage: Aliquot and store at -20°C.  Minimize freezing and thawing.
Recommended
Positive Control:
human brain or heart
 

Background
The androgen receptor (AR) is an ~110 kDa androgen-dependent transcription factor that is a member of the steroid/nuclear receptor gene superfamily.  The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain.  Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease and male infertility.   The PI3K/Akt signaling pathway plays an important role in regulating AR activity through phosphorylation of AR at Ser213/210 and Ser791/790.  Growth factors or cytokines may induce phosphorylation of AR through the PI3K/Akt pathway.  IGF-1 activates the  phosphatidylinositol 3-kinase(PI3K)/AKT pathway in LNCap at high passage number and increases phosphorylation of of  AR at Ser213/210 (can be detected using IMGENEX Cat #IMG-561) and Ser791/790 (1).  Activation of the PI3K/AKt pathway is thought to have a survival role in prostate cancer by protecting cells from apoptosis.

Antigen
Peptide with sequence EVQLGLGRVYPRPPSC, from N Terminus of the protein sequence according to NP_000035.

Application Notes
An ~95 kDa band is observed in human brain and heart  lysates (calculated MW of 99 kDa according to NP_000035).

 
Androgen Receptor Antibody

Staining of 35 µg of human brain lysate using IMG-3238 at 0.3 µg/ml.  Primary incubation was 1 hour.  Detected by chemiluminescence.

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Reference
1. Dong, KL and C Chang. 2003. Expression and degradation of androgen receptor: mechanism and clinical implication. J Clinical Endrocrinology & Metabolism. 88:4043-4054.
2. Trapman J, Klaassen P, Kuiper GG, van der Korput JA, Faber PW, van Rooij HC, Geurts van Kessel A, Voorhorst MM, Mulder E, Brinkmann AO. Cloning, structure and expression of a cDNA encoding the human androgen receptor. Biochem Biophys Res Commun. 153(1):241-248 (1998). PMID: 3377788


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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