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Polyclonal Antibody to Androgen Receptor |
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| Catalog No: |
IMG-3238 |
| Contents: |
0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Isotype: |
Goat Ig |
| Clone: |
N/A |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Human |
| Predicted React: |
Chimpanzee, Mouse, Rat |
| Host: |
Goat |
| Application: |
ELISA: >1:64000
Western blot analysis: 0.3-2 ug/ml |
| Storage: |
Aliquot and store at -20°C. Minimize freezing and thawing. |
Recommended
Positive Control: |
human brain or heart |
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Background
The androgen receptor (AR) is an ~110 kDa androgen-dependent transcription factor that is a member of the steroid/nuclear receptor gene superfamily. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease and male infertility. The PI3K/Akt signaling pathway plays an important role in regulating AR activity through phosphorylation of AR at Ser213/210 and Ser791/790. Growth factors or cytokines may induce phosphorylation of AR through the PI3K/Akt pathway. IGF-1 activates the phosphatidylinositol 3-kinase(PI3K)/AKT pathway in LNCap at high passage number and increases phosphorylation of of AR at Ser213/210 (can be detected using IMGENEX Cat #IMG-561) and Ser791/790 (1). Activation of the PI3K/AKt pathway is thought to have a survival role in prostate cancer by protecting cells from apoptosis. |
Antigen
Peptide with sequence EVQLGLGRVYPRPPSC, from N Terminus of the protein sequence according to NP_000035. |
Application Notes
An ~95 kDa band is observed in human brain and heart lysates (calculated MW of 99 kDa according to NP_000035). |
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Staining of 35 µg of human brain lysate using IMG-3238 at 0.3 µg/ml. Primary incubation was 1 hour. Detected by chemiluminescence. |
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Reference
1. Dong, KL and C Chang. 2003. Expression and degradation of androgen receptor: mechanism and clinical implication. J Clinical Endrocrinology & Metabolism. 88:4043-4054.
2. Trapman J, Klaassen P, Kuiper GG, van der Korput JA, Faber PW, van Rooij HC, Geurts van Kessel A, Voorhorst MM, Mulder E, Brinkmann AO. Cloning, structure and expression of a cDNA encoding the human androgen receptor. Biochem Biophys Res Commun. 153(1):241-248 (1998). PMID: 3377788
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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