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Polyclonal Antibody to 58K Golgi protein |
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| Catalog No: |
IMG-3239 |
| Contents: |
0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Isotype: |
Goat Ig |
| Clone: |
N/A |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Human |
| Predicted React: |
Mouse |
| Host: |
Goat |
| Application: |
IHC (paraffin): 3-5µg/ml
Western blot analysis: 0.03-0.5 µg/ml |
| Storage: |
Aliquot and store at -20°C. Minimize freezing and thawing. |
Recommended
Positive Control: |
human liver |
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Background
Principal Names – FTCD; LCHC1; formiminotransferase cyclodeaminase; formimidoyltransferase cyclodeaminase. Official Gene Symbol - FTCD. GenBank Accession Number – NP_006648; NP_996848. GeneID - 10841 (human). Gene Ontology terms - cytoplasm; folic acid and derivative metabolism; folic acid binding; formimidoyltetrahydrofolate cyclodeaminase activity; glutamate formimidoyltransferase activity; histidine metabolism; lyase activity; metabolism; transferase activity. This antibody is expected to recognise both reported isoforms (as represented by NP_006648 and NP_996848). |
Antigen
Peptide with sequence SQLVECVPNFSEGKNQ, from N Terminus of the protein sequence according to NP_006648; NP_996848. |
Application Notes
Western Blot: A 55-60kDa band observed in Human Liver lysate (calculated MW of 58.9kDa according to NP_006648. Recommended concentration: 0.03-0.5µg/ml.Immunohistochemistry: In paraffin embedded Human Liver shows distinct staining of membranes and cytoplasm in a selection of hepatocytes. Recommended concentration, 3-5µg/ml. |
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IMG-3239 (0.03 µg/ml) staining of human liver lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
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IHC staining of 58K Golgi protein in paraffin embedded Human Liver using IMG-3239 at 3.8µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
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Reference
1. Bloom GS, Brashear TA. A novel 58-kDa protein associates with the Golgi apparatus and microtubules. J Biol Chem. 1989 Sep 25;264(27):16083-92. PMID: 2777777
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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