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Product : Antibody >> Antigen Affinity Purified , New Antibodies , Nuclear & Cytoplasmic.. >> 58K Golgi protein
      
 

Polyclonal Antibody to 58K Golgi protein

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-3239
58K Golgi proteinPurified, Peptide Affinity
0.1 mg
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Catalog No: IMG-3239
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Isotype: Goat Ig
Clone: N/A
Purification: Antigen Affinity Chromatography
Species: Human
Predicted React: Mouse
Host: Goat
Application: IHC (paraffin): 3-5g/ml
Western blot analysis: 0.03-0.5 g/ml
Storage: Aliquot and store at -20C. Minimize freezing and thawing.
Recommended
Positive Control:
human liver
 

Background
Principal Names FTCD; LCHC1; formiminotransferase cyclodeaminase; formimidoyltransferase cyclodeaminase. Official Gene Symbol - FTCD. GenBank Accession Number NP_006648; NP_996848. GeneID - 10841 (human). Gene Ontology terms - cytoplasm; folic acid and derivative metabolism; folic acid binding; formimidoyltetrahydrofolate cyclodeaminase activity; glutamate formimidoyltransferase activity; histidine metabolism; lyase activity; metabolism; transferase activity. This antibody is expected to recognise both reported isoforms (as represented by NP_006648 and NP_996848).

Antigen
Peptide with sequence SQLVECVPNFSEGKNQ, from N Terminus of the protein sequence according to NP_006648; NP_996848.

Application Notes
Western Blot: A 55-60kDa band observed in Human Liver lysate (calculated MW of 58.9kDa according to NP_006648. Recommended concentration: 0.03-0.5g/ml.Immunohistochemistry: In paraffin embedded Human Liver shows distinct staining of membranes and cytoplasm in a selection of hepatocytes. Recommended concentration, 3-5g/ml.

 
58K Golgi protein Antibody

IMG-3239 (0.03 g/ml) staining of human liver lysate (35 g protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

58K Golgi protein Antibody IMG-3239

IHC staining of 58K Golgi protein in paraffin embedded Human Liver using IMG-3239 at 3.8g/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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Reference
1. Bloom GS, Brashear TA. A novel 58-kDa protein associates with the Golgi apparatus and microtubules. J Biol Chem. 1989 Sep 25;264(27):16083-92. PMID: 2777777


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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