Monoclonal Antibody against Human IL-17E (IL-25)

Background
A number of cytokines belonging to the interleukin (IL)-17 family have been identified, termed IL-17A-F.  IL-17 is a potent proinflammatory cytokine that plays roles in a number of diseases including rheumatoid arthritis (3), multiple sclerosis (4), and promotion of tumor growth (5). IL-17E, C, and B are able to induce proinflammatory responses. However, they do not bind to the IL-17 receptor suggesting that an additional IL-17R related receptor may exist. Receptors for IL-17B and IL-17E have been independently isolated by Shi, et al (6) and Lee, et al. (2) and have been designated as EV127 (in mouse) and IL-17Rh1 (in human), respectively. IL-17E, also called IL-25, induces activation of NF-kB pathway and like IL-17 also induces production of IL-8.

Antigen
Amino acids 115-132 of human IL-17E (isoform CRA_a) were used as immunogen for this antibody.

Reference
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2. Matusevicius D, Kivisakk P, He B, Kostulas N, Ozenci V, Fredrikson S, and Link H. Mult. Scler 5: 101-104 (1999).
3. Shalom-Barak T, Quach J, and Lotz M. J. Biol. Chem. 273: 27467-27473 (1998).
4. Lee J, Ho W-H, Maruoka M, Corpus RT, Baldwin DT, Fosters  JS, Goddard AD, Yansura DG, Vandlen RL, Wood WI, and Gurney AL. J. Biol. Chem. 276: 1660-1664 (2001).
5. Li H, Chen J, Huang A, Stinson J, Heldens S, Foster J, Dowd P, Gurney AL and wood WI. Proc. Natl. Acad. Sci., USA 97: 773-778 (2000).
6. Shi Y, Ulrich SJ, Zhang J, Connolly K., et al. J Biol. Chem. 275: 19167-19176 (2000).