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Monoclonal Antibody to AKT3 |
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| Catalog No: |
IMG-326 |
| Contents: |
100 µg in 200 µl PBS containing 0.05% sodium azide, 0.05% BSA. Sodium azide is highly toxic |
| Isotype: |
Mouse IgG1 |
| Clone: |
66C1247.1 |
| Purification: |
Protein G Chromatography |
| Species: |
Human, Mouse, Rat |
| Host: |
Mouse |
| Application: |
Western blot analysis: 1-2 ug/ml |
| Storage: |
Store at 4°C for six months. For long term storage, store at -20°C. |
Recommended
Positive Control: |
human kidney |
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Background
AKT, protein kinase B (PKB), is a serine/threonine kinase which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activat phosphtidylinositol 3-kinase (PI 3-kinase). The major activity of AKT/PKB is to mediate cell survival. AKT/PKB is also believed to be a critical factor in the genesis of cancer as the tumor suppressor PTEN was found to antagonise PI-3 kinase and AKT/PKB kinase activity. There are 3 known isoforms of this enzyme in mammalian cells (1/alpha, 2/beta and 3/gamma). AKT1, 2 and 3 contain a key regulatory serine phosphorylation site in the carboxy-terminal region of the protein. AKT/PKB phosphorylation is critical for it activity. |
Antigen
This monoclonal antibody was raised against a synthetic peptide corresponding to amino acid residues 119-136 (CSPTSQIDNIGEEEMDAS) of human AKT3, GenBank no. gi|4574744|gb|AAD24196.1|AF135794_1. This sequence is identical in human, mouse, rat, cow, dog, and chicken. |
Application Notes
A 50 kDa band is observed. |
Genebank Info (Protein)
NP_005456 |
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Western blot analysis for AKT3 using IMG-326 at 2 ug/ml dilution against 30 ug/lane of human kidney lysate (Imgenex cat. No. 40144). |
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Reference
1. Alessi DR., et al. EMBO J. 15: 6541-6551 (1996).
2. Ferring P., et al. Oncogene 18: 6129-6134 (1999).
3. Kandel ES, et al. Exp. Cell Res. 253: 210-219 (1999).
4. Cantley LC, et al. Proc Natl Acad Sci, USA, 96: 420-4245 (1999).
5. Franke TF, et al. Cell 88: 435-437 (1997).
6. Nakatani K, Sakaue H, et al. Biochem. Biophys. Res. Commun. 257 (3), 906-910 (1999)
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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