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Peptide-Affinity Purified Polyclonal Antibody to POLL/BETA-N |
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| Catalog No: |
IMG-3321 |
| Contents: |
0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Isotype: |
Goat Ig |
| Clone: |
N/A |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Human |
| Predicted React: |
Mouse |
| Host: |
Goat |
| Application: |
IHC (paraffin): 5-10µg/ml |
| Storage: |
Store at -20°C. |
Recommended
Positive Control: |
Human Small Intestine |
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Background
Principal Names – POLL; BETA-N; POL-KAPPA; polymerase (DNA directed), lambda; polymerase (DNA-directed), lambda; DNA polymerase kappa (DNA polymerase beta-N)
Official Gene Symbol - POLL
GenBank Accession Number – NP_037406
LocusLink ID - 27343 (human); 56626 (mouse) |
Antigen
Peptide with sequence LGLPYREPAERDW, from C Terminus of the protein sequence according to NP_037406. |
Application Notes
Peptide ELISA: antibody detection limit dilution >1:32,000. Western Blot: Preliminary experiments gave a band at approx 40kDa in Human Liver and HepG2 extracts at 1µg/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 66Kda according to NP_037406 (however please see comment above about Pol beta). Immunohistochemistry: In paraffin embedded Human Small Intestine shows nuclear staining of enterocytes and of most cells in the lamina propria. Recommended concentration, 5-10µg/ml. |
Genebank Info (Protein)
NP_037406 |
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IHC analysis of POLL/BETA-N in paraffin embedded Human Small Intestine using IMG-3321 at 5µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
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Reference
1. Aoufouchi S, Flatter E, Dahan A, Faili A, Bertocci B, Storck S, Delbos F, Cocea L, Gupta N, Weill JC, Reynaud CA. Two novel human and mouse DNA polymerases of the polX family. Nucleic Acids Res. 2000 Sep 15;28(18):3684-93. PMID: 10982892
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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