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Peptide-affinity Purified Polyclonal Antibody to ARP1 homolog A |
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| Catalog No: |
IMG-3360 |
| Contents: |
0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Isotype: |
Goat Ig |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Human |
| Predicted React: |
Human, Mouse, Rat |
| Host: |
Goat |
| Application: |
Western blot analysis: 1-3µg/ml |
| Storage: |
Store at -20°C. |
Recommended
Positive Control: |
human kidney lysate |
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Background
Principal Names – ACTR1A; ARP1; ARP1 actin-related protein 1 homolog A, centractin alpha (yeast); actin-RPV; centractin alpha; ARP1, yeast homolog A; centrosome-associated actin homolog; ARP1 (actin-related protein 1, yeast) homolog A (centractin alpha) Official Gene Symbol - ACTR1AGenBank Accession Number – NP_005727.1 Gene ID - 10121 (human); 54130 (mouse)Gene Ontology terms - vesicle-mediated transport; microtubule associated complex; dynactin complexThis antibody may crossreact with ACTR1B. |
Antigen
Peptide with sequence C-YEEDGARSIHRKT, from the C Terminus of the protein sequence according to NP_005727.1. |
Application Notes
Peptide ELISA: antibody detection limit dilution 1:1,000. Western Blot: Approx. 45kDa band observed in Human Kidney lysates (calculated MW of 42.6kDa according to NP_005727.1). Recommended concentration: 1-3µg/ml. |
Genebank Info (Protein)
NP_005727.1 |
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Western blot analysis of ARP1 homolog A in human kidney lysate using IMG-3360 at 2µg/ml (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
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Reference
1. Lees-Miller JP, Helfman DM, Schroer TA.A vertebrate actin-related protein is a component of a multisubunit complex involved in microtubule-based vesicle motility.Nature. 1992 Sep 17;359(6392):244-6. PMID: 1528266
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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