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Peptide-affinity Purified Polyclonal Antibody to ARP1 homolog B |
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| Catalog No: |
IMG-3361 |
| Contents: |
0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Isotype: |
Goat Ig |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Human |
| Predicted React: |
Mouse, Human, Rat |
| Host: |
Goat |
| Application: |
IHC (paraffin): 3-5µg/ml
Western blot analysis:0.5-2µg/ml
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| Storage: |
Store at -20°C. |
Recommended
Positive Control: |
human pancreas or testis |
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Background
Principal Names – ACTR1B; ARP1B; CTRN2; ARP1 actin-related protein 1 homolog B, centractin beta (yeast); PC3; centractin beta; ARP1, yeast homolog B; ARP1 (actin-related protein 1, yeast) homolog B (centractin beta) Official Gene Symbol - ACTR1BGenBank Accession Number – NP_005726 Gene ID - 10120 (human); 226977 (mouse)Gene Ontology terms - microtubule associated complex; dynactin complexThis antibody may crossreact with ACTR1A. |
Antigen
Peptide with sequence YEEDGSRAIHRKT, from C Terminus of the protein sequence according to NP_005726 |
Application Notes
Western Blot: Approx 45kDa band observed in human heart and brain lysates (predicted MW of 43kDa according to NP_005726). Recommended for use at 0.5-2µg/ml.Immunohistochemistry: In paraffin embedded Human Uterus shows fibrillar staining of cytoplasm in endometrial cells. Recommended concentration, 3-5µg/ml. |
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IMG-3361 staining (1µg/ml) of Human Heart lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
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IHC staining of ARP1 homolog B in paraffin embedded Human Uterus using IMG-3361 at 3.8µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
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Reference
1. Clark SW, Staub O, Clark IB, Holzbaur EL, Paschal BM, Vallee RB, Meyer DI.Beta-centractin: characterization and distribution of a new member of the centractin family of actin-related proteins.Mol Biol Cell. 1994 Dec;5(12):1301-10. PMID: 7696711
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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