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Peptide-affinity Purified Polyclonal Antibody to 58K Golgi protein |
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| Catalog No: |
IMG-3369 |
| Contents: |
0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Isotype: |
Goat Ig |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Human |
| Predicted React: |
Mouse |
| Host: |
Goat |
| Application: |
IF/ICC: See Hagiwara et al (2006) for further details.
IHC (paraffin): 0.01-0.03µg/ml
Western blot analysis:0.03-0.1µg/ml |
| Storage: |
Store at -20°C. |
Recommended
Positive Control: |
Human Liver lysate |
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Background
Principal Names – FTCD; LCHC1; formiminotransferase cyclodeaminase; formimidoyltransferase cyclodeaminase Official Gene Symbol - FTCDGenBank Accession Number – NP_006648; NP_996848 Gene ID - 10841 (human)Gene Ontology terms - cytoplasm; folic acid and derivative metabolism; folic acid binding; formimidoyltetrahydrofolate cyclodeaminase activity; glutamate formimidoyltransferase activity; histidine metabolism; lyase activity; metabolism; transferase activityThis antibody is expected to recognise both reported isoforms. |
Antigen
Peptide with sequence CLREQGRGKDQPGRL, from Internal of the protein sequence according to NP_006648; NP_996848 |
Application Notes
Western Blot: A 55-60kDa band observed in Human Liver lysates (calculated MW of 58.9kDa according to NP_006648 and NP_996848). Recommended concentration: 0.03-0.1µg/ml.Immunohistochemistry: In paraffin embedded Human Liver shows distinct staining of membranes and cytoplasm in a selection of hepatocytes. Recommended concentration, 3-5µg/ml. |
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IMG-3369 staining (0.01µg/ml) of Human Liver lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
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IHC staining of 58K Golgi protein in paraffin embedded Human Liver using IMG-3369 at 3.8µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
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Reference
1. Bloom GS, Brashear TA.A novel 58-kDa protein associates with the Golgi apparatus and microtubules.J Biol Chem. 1989 Sep 25;264(27):16083-92. PMID: 2777777
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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