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Product : Antibody >> Cancer Antibodies , Cell Cycle , DNA Methylation & Rep.. , Signal Transduction >> Chk2
      
 

Monoclonal Antibody to Chk2 (Checkpoint Kinase 2)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-336A
Chk2Purified
0.1 mg
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Catalog No: IMG-336A
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ug antibody in 200 ul PBS containing 0.2% gelatin and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Mouse IgG1
Clone: 73C175.1.1
Purification: Protein G Chromatography was used purify the antibody
Species: Human, Mouse
Host: Mouse
Application: Western blot analysis: use antibody at 2-4 ug/ml
Storage: Store Chk2 antibody at 4°C, stable for 6 months. For long-term storage, aliquot and store antibody at -20°C.
 

Background
Chk2 (checkpoint kinase 2) is the mammalian homolog of the Saccharomyces cerevisiae Rad53 and is required for the DNA damage and replication checkpoints (1). In response to low-dose ionizing radiation (IR), which occurs in an ataxia telangiectasia mutated (ATM)-dependent manner, Chk2 can phosphorylate the mitosis-inducing phosphatase Cdc25C on an inhibitory site, blocking entry into mitosis, and p53 on a regulatory site, causing G1 arrest (2,3). In human tissues, Chk2 is homogeneously expressed in renewing cell populations such as epidermis or intestine, heterogeneous in conditionally renewing tissues, and absent or at low level in static tissues such as muscle or brain (4). Mutations in Chk2 inactivates DNA damage checkpoint pathway involving Chk2 in lung cancer suggesting that reduced expression of Chk2 may be responsible for the development of lung cancer (5). The Chk2-/- knockout cells are resistant to DNA damage-induced apoptosis, defective for p53 stabilization and for induction of p53-dependent transcripts such as p21 in response to gamma irradiation (6).

Antigen
A portion of amino acids 100-150 of human Chk2 protein was used as immunogen for this antibody.

Genebank Info (Protein): NP_009125
Gene ID: 11200
SwissProt: O96017
 
Chk2 Antibody WB

Western blot analysis of Chk2 in A) human HEK293 and B) mouse NIH3T3 lysate using Chk2 antibody at 2 ug/ml.

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Reference
1. Lukas C, Bartkova J, Latella L, Falck J, Mailand N, Schroeder T, Sehested M, Lukas J, Bartek J. Cancer Res 61(13):4990-4993 (2001).
2. Shieh SY, Ahn J, Tamai K, Taya Y, Prives C. Genes Dev 14(3): 289-300 (2000). 
3. Falck J, Mailand N, Syljuasen RG, Bartek J, Lukas J.  Nature 410 (6830): 842-847 (2001).
4. Matsuoka S, Huang M, Elledge SJ. Science 4; 282(5395): 1893-1897 (1998).
5. Hirao A, Kong YY, Matsuoka S, Wakeham A, Ruland J, Yoshida H, Liu D, Elledge SJ, Mak TW. Science 10; 287(5459):1824-1827 (2000).
6. Matsuoka S, Nakagawa T, Masuda A, Haruki N, Elledge SJ, Takahashi T. Cancer Res 61(14):5362-5 (2001).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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