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Datasheet
Polyclonal Antibody to Histone deacetylase 4 (HDAC4)
Cat.No IMG-338
Description HDAC-4
Format Purified
Unit 0.1 mg
Price(USD) 225.00
MSDS
Catalog No
:
IMG-338
Contents
:
100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype
:
Rabbit IgG
Clone
:
N/A
Purification
:
Protein G Chromatography
Species
:
Human, Mouse
Host
:
Rabbit
Application
ChIP:Refer to Caretti et al (2003, Fig.3) and Imbriano (2005, Fig 7) for details. IP: User should empirically determine optimal conditions Western blot analysis: 0.5-2 ug/ml Storage Store at 4oC, stable for 6 months. For long-term storage, store at -20oC. Recommended Positive Control : Jurkat or NIH 3T3
Background Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins (1). Eight members of HDAC family have been identified in the past several years (2,3). These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3). These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.
Antigen
This antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acids 194-209 of human HDAC4.
Reference 1. Cress, W.D. and Seto, E. J. Cell. Physiol. 184, 1-16 (2000). 2. Meinke PT and Liberator P. Curr Med Chem, 8(2): 211-235 (2001). 3. Nakayama T and Takami Y. J Biochem (Tokyo) 2001, 129 (4): 491-499 (20001).
Product Citations 1. Dynamic recruitment of NF-Y and histone acetyltransferases on cell-cycle promoters. Caretti G, V Salsi , C Vecchi, C Imbriano, and R Mantovani. J BiolChem. 2005:30435-30440. (ChIP, Fig. 3) 2. Direct p53 Transcriptional Repression: In vivo analysis of CCAAT-containing G2/M promoters. Imbriano C, A Gurtner, F Cocchiarella, S Di Agostino, V Basile, M Gostissa, M Dobbelstein, G Del Sal, G Piaggion, and R Mantovani. Mol Cell Biol. 25:3737-3751. (ChIP, Fig. 7) 3. HDAC4 promotes growth of colon cancer cells via repression of p21. Wilson A, D Byun, S Nasser, L Murray, K Ayyanar, D Arango, M Figueroa, A Melnick, G Kao, L Augenlicht, J Mariadason. MBC: 10.1091/mbc.E08-02-0139 (2008). Imgenex antibodies cited (human HTC116 cells): 1. HDAC1 (IMG-337): WB: Figs. 2A,B; 5D. 2. HDAC4 (IMG-338): WB: Figs. 1A, E, F, 4C, 5D, 6A, 7B; ChIP: Figs. 10A,C,D,H.
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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