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Peptide-affinity Purified Polyclonal Antibody to AKT3 |
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| Catalog No: |
IMG-3389 |
| Contents: |
0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Isotype: |
Goat Ig |
| Clone: |
N/A |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Human |
| Predicted React: |
Mouse, Rat |
| Host: |
Goat |
| Application: |
Western blot analysis:1µg/ml |
| Storage: |
Store at -20°C. |
Recommended
Positive Control: |
HepG2 lysate |
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Background
Principal Names – AKT-3; PKBG; PRKBG; STK-2; RAC-gamma; RAC-PK-gamma; DKFZP434N0250; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma); protein kinase B gamma; serine threonine protein kinase, AKT-3; RAC-gamma serine/threonine protein kinase Official Gene Symbol - AKT-3 GenBank Accession Number – NP_005456; NP_859029 Gene ID - 10000 (human)Gene Ontology terms - ATP binding; protein amino acid phosphorylation; protein serine/threonine kinase activity; signal transduction; transferase activityThis antibody is expected to recognise an epitope corresponding to aa 119-133 of both isoforms of human AKT-3 portein. This antibody does not cross-react with human AKT1/2. |
Antigen
Peptide with sequence CSPTSQIDNIGEEEM, from Internal of the protein sequence according to NP_005456; NP_859029 |
Application Notes
Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: A 50-55kDa band observed in Human Hepatoblastoma HepG2 lysates (calculated MW of 55.8kDa according to NP_005456 and 54.0kDa according to NP_859029). Recommended concentration: 1-3µg/ml. |
Genebank Info (Protein)
NP_005456 |
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IMG-3389 (1µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
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HEK293 overexpressing AKT3 with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and using IMG-3389 in the right panel (vector-only transfection in first and fourth lanes). |
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Reference
1. Zinda MJ, Johnson MA, Paul JD, Horn C, Konicek BW, Lu ZH, Sandusky G, Thomas JE, Neubauer BL, Lai MT, Graff JR.
AKT-1, -2, and -3 are expressed in both normal and tumor tissues of the lung, breast, prostate, and colon.
Clin Cancer Res. 2001 Aug;7(8):2475-9.
PMID: 11489829
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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