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Product : Antibody >> Cancer Antibodies , Chromatin , DNA Methylation & Rep.. , Histone Deacetylases >> HDAC-5
      
 

Polyclonal Antibody to Histone Deacetylase 5 (HDAC-5)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-339
HDAC-5 Purified
0.1 mg
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Catalog No: IMG-339
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 g in 200 l PBS containining 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit IgG
Purification: Protein G Chromatography
Species: Human, Mouse
Host: Rabbit
Application: ChIP: Refer to Imbriano (2005, Fig 7) for details.
IP: User should empirically determine optimal conditions
Western blot analysis: 1-3 ug/ml
Storage: Store at 4oC, stable for 6 months. For long-term storage, store at -20oC.
 

Background
Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins (1). Eight members of HDAC family have been identified in the past several years (2,3). These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3). These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.

Antigen
This antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acids 572-587 of human HDAC-5.

Genebank Info (Protein): NP_001015053
Gene ID: 10014
SwissProt: Q9UQL6
 
HDAC-5 Antibody

Western blot analysis of HDAC-5 in NIH 3T3 cell lysate using IMG-339 at 2 ug/ml.

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Reference
1. Cress, W.D. and Seto, E. J. Cell. Physiol. 184, 1-16 (2000).
2. Meinke PT and Liberator P. Curr Med Chem, 8(2):211-235 (2001).
3. Nakayama T and Takami Y. J Biochem (Tokyo) 2001, 129 (4): 491-499 (20001).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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