PATHWAY PROFILING ANTIBODIES & REAGENTS

     
Products
Product : Antibody >> Cell Cycle , Signal Transduction >> Aurora kinase A/STK15
      
 

Peptide-affinity Purified Polyclonal Antibody to Aurora Kinase A

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-3406
Aurora kinase A/STK15Purified, Peptide Affinity
0.1 mg
 --
 
Catalog No: IMG-3406
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Isotype: Goat IgG
Clone: N/A
Purification: Antigen Affinity Chromatography
Species: Human
Predicted React: Human
Host: Goat
Application: Western Blot: 0.1-0.3g/ml
Storage: Store at -20C. For long term storage, store at -70C
Recommended
Positive Control:
Jurkat lysate
 

Background
Principal Names: AURKA; aurora kinase A; AIK; ARK1; AURA; AURORA2; BTAK; MGC34538; STK15; STK6; STK7; IPL1-related kinase; aurora-A; aurora-related kinase 1; aurora/IPL1-like kinase; breast-tumor-amplified kinase; serine/threonine kinase 15; serine/threonine kinase 6; serine/threonine protein kinase 15; serine/threonine protein kinase 6; Official Symbol: AURKA; Accession Number(s): NP_003591.2; Human GeneID(s): 6790; Gene Ontology Terms: cAMP-dependent protein kinase activity; protein kinase CK2 activity; protein tyrosine kinase activity; ATP binding; protein serine/threonine kinase activity; cell cycle; mitosis; protein amino acid phosphorylation; oncogenesis; spindle; spindle pole body; nucleus; transferase activity

Antigen
Peptide with sequence C-QNKESASKQS, from the C Terminus of the protein sequence according to NP_003591.2.

Application Notes
Western Blot: Approx 45kDa band observed in lysates of cell line HeLa (calculated MW of 45.8kDa according to NP_003591.2). Recommended concentration: 0.1-0.3g/ml.

 
Aurora kinase A/STK15 Antibody

Western blot analysis of Aurora kinase A/STK15 using IMG-3406 in Jurkat lysate (35g protein in RIPA buffer) at 0.1g/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Aurora kinase A/STK15 Antibody IMG-3406

HEK293 overexpressing Aurora kinase A with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and using IMG-3406 in the right panel (vector-only transfection in first and fourth lanes).

-

Reference
1. Zhou H, Kuang J, Zhong L, Kuo WL, Gray JW, Sahin A, Brinkley BR, Sen S. Tumour amplified kinase STK15/BTAK induces centrosome amplification, aneuploidy and transformation. Nat Genet 1998 Oct;20(2):189-93 PMID: 9771714


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


Products

Services

Resources

Purchasing

Company

  • What's New
  • Reporter Cells
  • Screening Services
  • Interactive Pathways