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Peptide-affinity Purified Polyclonal Antibody to Aurora Kinase A |
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| Catalog No: |
IMG-3406 |
| Contents: |
0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Isotype: |
Goat IgG |
| Clone: |
N/A |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Human |
| Predicted React: |
Human |
| Host: |
Goat |
| Application: |
Western Blot: 0.1-0.3µg/ml |
| Storage: |
Store at -20°C. For long term storage, store at -70°C |
Recommended
Positive Control: |
Jurkat lysate |
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Background
Principal Names: AURKA; aurora kinase A; AIK; ARK1; AURA; AURORA2; BTAK; MGC34538; STK15; STK6; STK7; IPL1-related kinase; aurora-A; aurora-related kinase 1; aurora/IPL1-like kinase; breast-tumor-amplified kinase; serine/threonine kinase 15; serine/threonine kinase 6; serine/threonine protein kinase 15; serine/threonine protein kinase 6; Official Symbol: AURKA; Accession Number(s): NP_003591.2; Human GeneID(s): 6790; Gene Ontology Terms: cAMP-dependent protein kinase activity; protein kinase CK2 activity; protein tyrosine kinase activity; ATP binding; protein serine/threonine kinase activity; cell cycle; mitosis; protein amino acid phosphorylation; oncogenesis; spindle; spindle pole body; nucleus; transferase activity |
Antigen
Peptide with sequence C-QNKESASKQS, from the C Terminus of the protein sequence according to NP_003591.2. |
Application Notes
Western Blot: Approx 45kDa band observed in lysates of cell line HeLa (calculated MW of 45.8kDa according to NP_003591.2). Recommended concentration: 0.1-0.3µg/ml. |
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Western blot analysis of Aurora kinase A/STK15 using IMG-3406 in Jurkat lysate (35µg protein in RIPA buffer) at 0.1µg/ml. Primary incubation was 1 hour. Detected by chemiluminescence. |
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HEK293 overexpressing Aurora kinase A with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and using IMG-3406 in the right panel (vector-only transfection in first and fourth lanes). |
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Reference
1. Zhou H, Kuang J, Zhong L, Kuo WL, Gray JW, Sahin A, Brinkley BR, Sen S.
Tumour amplified kinase STK15/BTAK induces centrosome amplification, aneuploidy and transformation.
Nat Genet 1998 Oct;20(2):189-93
PMID: 9771714
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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