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Product : Antibody >> Chromatin , DNA Fragmentation , DNA Methylation & Rep.. >> APE1/APEX1
      
 

Peptide-affinity Purified Polyclonal Antibody to APE1/APEX1

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-3470
APE1/APEX1Purified, Peptide Affinity
0.1 mg
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Catalog No: IMG-3470
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Isotype: Goat Ig
Clone: N/A
Purification: Antigen Affinity Chromatography
Species: Human
Host: Goat
Application: IHC (p): 4-6g/ml.
Western blot analysis: 0.1-0.3g/ml.
Storage: Store at -20C.
Recommended
Positive Control:
Human lysates of cell lines A431 and HeLa
 

Background
Principal Names: APEX; APEX1; APEX nuclease (multifunctional DNA repair enzyme); APE; APX; APE1; APEN; HAP1; REF1; REF-1; AP lyase; AP endonuclease class I; apurinic/apyrimidinic exonuclease; multifunctional DNA repair enzyme; DNA-(apurinic or apyrimidinic site) lyase; apurinic/apyrimidinic (abasic) endonuclease; deoxyribonuclease (apurinic or apyrimidinic); Official Symbol: APEX1; Accession Number(s): NP_001632.2; NP_542379.1; NP_542380.1; Human GeneID(s): 328; Gene Ontology Terms: DNA-(apurinic or apyrimidinic site) lyase activity; uracil DNA N-glycosylase activity; endodeoxyribonuclease activity; transcription co-repressor activity; 3'-5' exonuclease activity; endonuclease activity; DNA binding; transcription co-activator activity; base-excision repair; DNA repair; transcription from Pol II promoter; obsolete; nucleus; Important Comments: NP_001632.2; NP_542379.1 and NP_542380.1 are varients that represent the same protein.

Antigen
Peptide with sequence PKRGKKGAVAEDGD-C, from the N Terminus of the protein sequence according to NP_001632.2.

Application Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western blot: Approx 35-37kDa band observed in Human lysates of cell lines A431 and HeLa (calculated MW of 35.6kDa according to NP_001632.2, NP_542379.1 and NP_542380.1). Recommended concentration: 0.1-0.3g/ml.IHC: In paraffin embedded Human Breast shows nuclear staining of duct epithelial cells. Recommended concentration, 4-6g/ml.

Genebank Info (Protein): NP_001632.2
Gene ID: 328
 
APE1/APEX1 Antibody

IMG-3470  (0.1g/ml) staining of A431 lysate (35g protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

APE1/APEX1

(4g/ml) IHC staining of paraffin embedded Human Breast. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

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Reference
1. Demple B, Herman T, Chen DS. Cloning and expression of APE, the cDNA encoding the major human apurinic endonuclease: definition of a family of DNA repair enzymes. Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11450-4. PMID: 1722334


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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