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Peptide-affinity Purified Polyclonal Antibody to APE1/APEX1 |
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| Catalog No: |
IMG-3470 |
| Contents: |
0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Isotype: |
Goat Ig |
| Clone: |
N/A |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Human |
| Host: |
Goat |
| Application: |
IHC (p): 4-6µg/ml.
Western blot analysis: 0.1-0.3µg/ml. |
| Storage: |
Store at -20°C. |
Recommended
Positive Control: |
Human lysates of cell lines A431 and HeLa |
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Background
Principal Names: APEX; APEX1; APEX nuclease (multifunctional DNA repair enzyme); APE; APX; APE1; APEN; HAP1; REF1; REF-1; AP lyase; AP endonuclease class I; apurinic/apyrimidinic exonuclease; multifunctional DNA repair enzyme; DNA-(apurinic or apyrimidinic site) lyase; apurinic/apyrimidinic (abasic) endonuclease; deoxyribonuclease (apurinic or apyrimidinic); Official Symbol: APEX1; Accession Number(s): NP_001632.2; NP_542379.1; NP_542380.1; Human GeneID(s): 328; Gene Ontology Terms: DNA-(apurinic or apyrimidinic site) lyase activity; uracil DNA N-glycosylase activity; endodeoxyribonuclease activity; transcription co-repressor activity; 3'-5' exonuclease activity; endonuclease activity; DNA binding; transcription co-activator activity; base-excision repair; DNA repair; transcription from Pol II promoter; obsolete; nucleus; Important Comments: NP_001632.2; NP_542379.1 and NP_542380.1 are varients that represent the same protein. |
Antigen
Peptide with sequence PKRGKKGAVAEDGD-C, from the N Terminus of the protein sequence according to NP_001632.2. |
Application Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western blot: Approx 35-37kDa band observed in Human lysates of cell lines A431 and HeLa (calculated MW of 35.6kDa according to NP_001632.2, NP_542379.1 and NP_542380.1). Recommended concentration: 0.1-0.3µg/ml.IHC: In paraffin embedded Human Breast shows nuclear staining of duct epithelial cells. Recommended concentration, 4-6µg/ml. |
Genebank Info (Protein)
NP_001632.2 |
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IMG-3470 (0.1µg/ml) staining of A431 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
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(4µg/ml) IHC staining of paraffin embedded Human Breast. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. |
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Reference
1. Demple B, Herman T, Chen DS.
Cloning and expression of APE, the cDNA encoding the major human apurinic endonuclease: definition of a family of DNA repair enzymes.
Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11450-4.
PMID: 1722334
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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