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Monoclonal Antibody to Noxa |
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| Catalog No: |
IMG-349A |
| Contents: |
100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Mouse IgG1, Kappa |
| Clone: |
114C307.1 |
| Purification: |
Protein G Chromatography |
| Species: |
Human, Mouse |
| Host: |
Mouse |
| Application: |
IF/ICC: See Yakovlev et all (2003) for details.
IHC (paraffin): 1:2000
Western blot analysis: 1-2 ug/ml |
| Storage: |
Store at 4°C for six months. For long term storage, store at -20°C. |
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Background
Recently, a BH-3 only member of the Bcl-2 family have been identified in human and mouse and designated as Noxa (for damage) (1). The expression of the Noxa gene involves direct activation of its promoter by p53. Increased expression of Noxa protein occurs in normal thymocytes but not in p53-deficient thymocytes. Noxa cDNA codes for a 103-amino acid protein. The coimmunoprecipitation data suggest that Noxa protein may interact with proteins belonging to the Bcl-2 family, such as, Bcl-XL and Mcl-1. Oda et al (1) have also shown that blocking the endogenous Noxa induction results in the suppression of apoptosis. Treatment of cells with Noxa antisense oligonucleotides blocks radiation-induced apoptosis suggesting that Noxa may represent a mediator of p53-dependent apoptosis. |
Antigen
Noxa Antibody was developed by immunizing mice with a fusion protein containing human Noxa. |
Application Notes
An ~80 kD background band may also be seen in Jurkat cell lysate. |
Genebank Info (Protein)
NP_066950 |
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Western blot detection of Noxa antibody in A) 293 cells transfected with human Noxa, B) RL-7 cell (a follicular lymphoma) and C) T98G lysate using IMG-349A at 2 µg/ml. |
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Bouin-fixed, paraffin-embedded Chronic Lymphocytic Leukemia (CLL) xenograft stained with Noxa antibody (1:2000), peroxidase-conjugate and DAB chromogen. Staining for 2 hr at RT was used. |
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Reference
1. Oda E, Ohki R, Murasawa H, Nemoto J, Shibue T,Yamashita T, Tokino, T, Taniguchi T, and Tanaka N. Science 288: 1053-1058 (2000).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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.jpg "Noxa Antibody IHC")
