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Datasheet
Monoclonal Antibody to Phosphorylated ATF2 (Thr71)
Cat.No IMG-350A
Description ATF2 (Thr71)
Unit 0.1 mg
Price(USD) 245.00
MSDS
Catalog No
:
IMG-350A
Contents
:
100 ug in 200 ul PBS containing 0.2% gelatin, 0.05% sodium azide. Sodium azide is highly toxic
Isotype
:
Mouse IgG1
Clone
:
103C411.2
Purification
:
Protein G Chromatography
Species
:
Human
Host
:
Mouse
Application
Western blot analysis: 2 ug/ml Storage This antibody is supplied as protein-G purified protein. Store at 4°C, stable at 4°C for 6 months. For long-term storage, store at -20°C. Recommended Positive Control : TNF-a treated HeLa (1 hr)
Background
ATF-2 is a member of the group of bZip transcription factors. Heterodimer formation between members of the bZip group is common and is believed to add diversity to the cis-acting elements at which binding of the dimers is directed. Specifically, ATF-2 may dimerize with c-Jun, as occurs in response to E1a, and in so doing shift the binding preference of c-Jun toward ATF/CRE sites (1-3). Deletion analysis has indicated that the N-terminal region of ATF-2 containing threonine at residues 69 and 71 are essential for this purpose. These threonine residues are phosphorylated by JNK/SAPK for transcriptional activation.
Antigen
This antibody was generated by immunizing mice with a synthetic peptide containing phosphorylated threonine at position 71 of human ATF-2.
Detection of ATF-2 phosphorylation in HeLa cell lysate with anti-phospho ATF-2 mAb (Cat. #IMG-350). Lane A. Untreated cell lysate; Lane B. HeLa cells treated with TNF for 1 hr.
Reference 1. Benbrook DM., and Jones NC. Oncogene 5, 295-302 (1990). 2. Hai T and Curran T. Proc. Natl. Acad. Sci. U. S. A. 88, 3720-3724 (1991). 3. van Dam, H., Duyndam, M., Rottier, R., Bosch, A., de Vries-Smits, L., Herrlich, P., Zantema, A., Angel, P., and van der Eb, A. J. (1993) EMBO J. 12, 479-487 (1993).
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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