Monoclonal Antibody to Phospho ATF2 (Thr71)

Background
ATF2 is a member of the group of bZip transcription factors. Heterodimer formation between members of the bZip group is common and is believed to add diversity to the cis-acting elements at which binding of the dimers is directed. Specifically, ATF2 may dimerize with c-Jun, as occurs in response to E1a, and in so doing shift the binding preference of c-Jun toward ATF/CRE sites (1-3). Deletion analysis has indicated that the N-terminal region of ATF2 containing threonine at residues 69 and 71 are essential for this purpose. These threonine residues are phosphorylated by JNK/SAPK for transcriptional activation.

Antigen
This antibody was generated by immunizing mice with a synthetic peptide containing phosphorylated threonine at position 71 of human ATF2.

Application Notes
This monoclonal antibody is specific for phospho (Thr71) ATF2. It cannot be used to test for non-phosphorylated ATF2.

Genebank Info (Protein)
NP_001871

Reference
1. Benbrook DM., and Jones NC. Oncogene 5, 295-302 (1990).
2. Hai T and Curran T. Proc. Natl. Acad. Sci. U. S. A. 88, 3720-3724 (1991).
3. van Dam, H., Duyndam, M., Rottier, R., Bosch, A., de Vries-Smits, L., Herrlich, P., Zantema, A., Angel, P., and van der Eb, A. J. (1993) EMBO J. 12, 479-487 (1993).