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Product : Antibody >> DNA Methylation & Rep.. , Neurobiology , Phosphospecific Antibodie.. , Transcription Factors >> ATF2 (Thr71)
      
 

Monoclonal Antibody to Phospho ATF2 (Thr71)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-350A
ATF2 (Thr71)Purified
0.1 mg
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Catalog No: IMG-350A
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ug antibody in 200 ul PBS containing 0.05% BSA, 0.05% sodium azide. Sodium azide is highly toxic
Isotype: Mouse IgG1
Clone: 103C411.2
Purification: Protein G Chromatography
Species: Human, Mouse
Host: Mouse
Application: Western blot analysis: 2 ug/ml
Storage: Store ATF2 antibody at 4°C, stable for 6 months. For long-term storage, store antibody at -20°C.
 

Background
ATF2 is a member of the group of bZip transcription factors. Heterodimer formation between members of the bZip group is common and is believed to add diversity to the cis-acting elements at which binding of the dimers is directed. Specifically, ATF2 may dimerize with c-Jun, as occurs in response to E1a, and in so doing shift the binding preference of c-Jun toward ATF/CRE sites (1-3). Deletion analysis has indicated that the N-terminal region of ATF2 containing threonine at residues 69 and 71 are essential for this purpose. These threonine residues are phosphorylated by JNK/SAPK for transcriptional activation.

Antigen
This antibody was generated by immunizing mice with a synthetic peptide containing phosphorylated threonine at position 71 of human ATF2.

Application Notes
This monoclonal antibody is specific for phospho (Thr71) ATF2. It cannot be used to test for non-phosphorylated ATF2.

Genebank Info (Protein): NP_001871
Gene ID: 1386
SwissProt: P15336
 
ATF2 (Thr71) Antibody

Detection of ATF2 phosphorylation in HeLa cell lysate with phospho ATF2 antibody. Lane A. Untreated cell lysate; Lane B. HeLa cells treated with TNF for 1 hr.

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Reference
1. Benbrook DM., and Jones NC. Oncogene 5, 295-302 (1990).
2. Hai T and Curran T. Proc. Natl. Acad. Sci. U. S. A. 88, 3720-3724 (1991).
3. van Dam, H., Duyndam, M., Rottier, R., Bosch, A., de Vries-Smits, L., Herrlich, P., Zantema, A., Angel, P., and van der Eb, A. J. (1993) EMBO J. 12, 479-487 (1993).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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