Peptide-affinity Purified Polyclonal Antibody to Hsp22/ Heat Shock Protein 22 (H11)

Background
Principal Names: H11; E2IG1; HSP22; HspB8; protein. kinase H11; small stress protein-like protein HSP22. Official Symbol: H11 (interim symbol). GenBank Accession Number(s): NP_055180. GeneID: 26353 (human). Gene Ontology Terms: protein serine/threonine kinase activity; heat shock protein activity; biological process unknown; cellular component unknown; transferase activity.

Antigen
Peptide with sequence NELPQDSQEVTCT, from the C Terminus of the protein sequence according to NP_055180.

Application Notes
Peptide ELISA: antibody detection limit dilution >1:32,000. Western Blot: Approx 20-25kDa band seen in Human Muscle lysate [Predicted MW of approx. 22kDa according to NP_055180]. Recommended for use at 0.5-1µg/ml.

Reference
1. Benndorf R, Sun X, Gilmont RR, Biederman KJ, Molloy MP, Goodmurphy CW, Cheng H, Andrews PC, Welsh MJ. HSP22, a new member of the small heat shock protein superfamily, interacts with mimic of phosphorylated HSP27 ((3D)HSP27). J Biol Chem. 2001 Jul 20;276(29):26753-61. Epub 2001 May 07. PMID: 11342557