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Peptide-affinity Purified Polyclonal Antibody to PHAPI2/APRIL |
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| Catalog No: |
IMG-3547 |
| Contents: |
0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Isotype: |
Goat Ig |
| Clone: |
N/A |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Human |
| Predicted React: |
Mouse, Rat |
| Host: |
Goat |
| Application: |
Western blot analysis: 0.3-1µg/ml. |
| Storage: |
Store at -20°C. |
Recommended
Positive Control: |
A431 lysate |
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Background
Principal Names: ANP32B; APRIL; SSP29; PHAPI2; acidic (leucine-rich) nuclear phosphoprotein 32 family, member B; acidic protein rich in leucines. Official Symbol: ANP32B. GenBank Accession Number(s): NP_006392. GeneID: 10541 (human); 67628 (mouse); 170724 (rat). Important Comments: This antibody does NOT recognize theTNF family member also known as APRIL - LocusLink Number 8741. |
Antigen
Peptide with sequence CKRKRETDDEGEDD, from the C Terminus of the protein sequence according to NP_006392. |
Application Notes
Peptide ELISA: antibody detection limit dilution 1:2,000. Western blot: Approx 26kDa band observed in Human Lymph Node lysates and in lysates of cell line A431 (calculated MW of 28.8kDa according to NP_006392.1). Recommended concentration: 0.3-1µg/ml. |
Genebank Info (Protein)
NP_006392 |
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Western blot analysis of PHAPI2/APRIL in A431 lysate (35µg protein in RIPA buffer) using IMG-3547 at 0.3µg/ml. Primary incubation was 1 hour. Detected by chemiluminescence. using chemiluminescence. |
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Reference
1. Zhu L, Perlaky L, Henning D, Valdez BC.
Cloning and characterization of a new silver-stainable protein SSP29, a member of the LRR family.
Biochem Mol Biol Int. 1997 Aug;42(5):927-35.
PMID: 9285060
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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