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Peptide-affinity Purified Polyclonal Antibody to KPNA4/Importin alpha 3 |
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| Catalog No: |
IMG-3569 |
| Contents: |
0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Isotype: |
Goat Ig |
| Clone: |
N/A |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Human |
| Host: |
Goat |
| Application: |
IHC (paraffin): 1-2µg/ml
Western blot analysis: 0.2-1µg/ml |
| Storage: |
Store at -20°C. |
Recommended
Positive Control: |
Human Testis lysate |
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Background
Principal Names: KPNA4; QIP1; SRP3; MGC12217; MGC26703; karyopherin alpha 4 (importin alpha 3); importin alpha 3. Official Symbol: KPNA4. GenBank Accession Number(s): NP_002259. GeneID: 3840 (human). Gene Ontology Terms: NLS-bearing substrate-nucleus import; importin, alpha-subunit. |
Antigen
Peptide with sequence CNSSANVPTEGFQF, from the C Terminus of the protein sequence according to NP_002259 |
Application Notes
Peptide ELISA: antibody detection limit dilution 1/32,000. Western Blot: Approx 55-60kDa band observed in Human Testis and Ovary lysates (predicted size of approx. 58kDa according to NP_002259.1). Recommended for use at 0.2-1µg/mlImmunohistochemistry: In paraffin embedded Human Testis, shows strong nuclear staining of some cells in the seminiferous tubules. Recommended concentration, 3µg/ml.Immunohistochemistry: In paraffin embedded Human Small Intestine shows pixulate staining in nuclei of enterocytes. Recommended concentration, 1-2µg/ml. |
Genebank Info (Protein)
NP_002259 |
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Western blot analysis of KPNA4 / Importin alpha 3 in Human Testis lysate using IMG-3569 at 0.5 ug/ ml. |
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IHC analysis of KPNA4/Importin alpha 3 using IMG-3569; Figure 1: paraffin embedded Human Testis at 3µg/ml. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining. Figure 2: paraffin embedded Human Small Intestine at 5µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
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Reference
1. Seki T, Tada S, Katada T, Enomoto T.
Cloning of a cDNA encoding a novel importin-alpha homologue, Qip1: discrimination of Qip1 and Rch1 from hSrp1 by their ability to interact with DNA helicase Q1/RecQL.
Biochem Biophys Res Commun. 1997 May 8;234(1):48-53.
PMID: 9168958
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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