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Peptide-affinity Purified Polyclonal Antibody to VPS35/MEM3 |
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| Catalog No: |
IMG-3575 |
| Contents: |
0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Isotype: |
Goat Ig |
| Clone: |
N/A |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Human |
| Predicted React: |
Mouse, Rat |
| Host: |
Goat |
| Application: |
IF/ICC: See Tabuchi et al (2010) for details.
IHC (paraffin):1µg/ml, Western blot analysis: 1-3µg/ml
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| Storage: |
Store at -20°C. |
Recommended
Positive Control: |
Human Brain lysate |
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Background
Principal Names: VPS35; MEM3; FLJ10752; FLJ13588; FLJ20388; DKFZp434E1211; DKFZp434P1672; vacuolar protein sorting 35 (yeast); maternal-embryonic 3. Official Symbol: VPS35. GenBank Accession Number(s): NP_060676. GeneID: 55737 (human); 65114 (mouse). Important Comments: Note there is a hypothetical protein called similar to vacuolar protein sorting 35 (XP_040192.1), which is virtually identical. |
Antigen
Peptide with sequence CSPESEGPIYEGLIL, from the C Terminus of the protein sequence according to NP_060676. |
Application Notes
Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 90kDa band observed in Human Brain and Testis lysates (predicted MW of 91kDa according to NP_060676.2). Recommended for use at 1-3µg/ml.
Immunohistochemistry: In paraffin embedded Human Testis, shows positive staining in Leydig cells, and cytoplasmic staining (dot-like) of some epithelial cells of the epididymis and of the rete testis. |
| Genebank Info (Protein): NP_060676 |
| Gene ID: 55737 |
| SwissProt: Q96QK1 |
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Western blot analysis of VSP35/MEM3 in Human Brain lysate using IMG-3575 at 1 ug/ ml. |
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IMG-3575 staining of paraffin embedded Human Testis showing A) Epithelial cells of the epididymis and B) Some leydig cells. Microwaved antigen retrieval with A)Tris/EDTA buffer pH9 at 3µg/ml or B)Citrate buffer pH6 at 10ug/ml., HRP-staining. |
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Reference
1. Zhang P, Yu L, Gao J, Fu Q, Dai F, Zhao Y, Zheng L, Zhao S.
Cloning and characterization of human VPS35 and mouse Vps35 and mapping of VPS35 to human chromosome 16q13-q21.
Genomics. 2000 Dec 1;70(2):253-7.
PMID: 11112353
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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