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Polyclonal Antibody to Histone-H2B (CT) |
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| Catalog No: |
IMG-359 |
| Contents: |
100 µg in 200 µl PBS containing 0.05% BSA, 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Purification: |
Protein G Chromatography |
| Species: |
Chicken, Dog, Drosophila, Human, Most Mammals, Mouse, Rat, Zebrafish |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 2 ug/ml |
| Storage: |
Store at 4oC, stable for 6 months. For long-term storage, store at -20oC. |
Recommended
Positive Control: |
Jurkat, PBMC |
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Background
Histones are relatively small proteins that contain a high proportion of positively charged amino acids, e.g., arginine and lysine. This positive charge allows histones to bind tightly to the negatively charged DNA. There are five basic types of histone molecules called H1, H2A, H2B, H3, and H4. Histones are abundantly present in virtually all eukaryotic cells. Human histone H2B is a 125 amino acid protein. |
Antigen
This antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acid residues 111-125 AVSEGTKAVTKYTSS of human Histone H2B; GenBank no. CAI19747.1. This peptide sequence is 100% conserved between multiple species including human, mouse, rat, dog, chimpanzee, cow, chicken, duck, zebrafish, and drosophila. |
Application Notes
A 15 kDa band is observed. The antibody is routinely quality controlled in human lysates. |
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Western blot analysis of Histone H2B in human PBMC lysate with IMG-359. |
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Western blot analysis of fractionated Cos-7 cells using IMG-359 anti-Histone H2B. Cos-7 cells were fractionated into nuclear cytosolic/membrane fractions. Approximately ~15ug of each fraction was separated on SDS PAGE. Lane 1, Nuclear fraction. Lane 2, Cytosolic/membrane fraction. |
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Western blot analysis of Histone H2B using IMG-359 at 1:250 in nuclear and cytoplasmic fractions made from Drosophila head extracts using IMGENEX's Nuclear Extraction Kit (10081K). Data courtesy of Dr. Jerry Yin, University of Wisconsin-Madison. |
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Reference
1. McGhee JD and Felsenfeld G. Ann Rev Biochem 49: 1115-1156 (1980).
2. Igo-Kemenes T, Horz W, Zachau HG. Ann Rev Biochem 51: 89-121 (1982).
3. Modesti M, Kanaar R. Curr Biol 2001 Mar 20;11(6):R229-32.
4. Nowak SJ, Corces VG. Genes Dev 14(23): 3003-13 (2000).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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