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Polyclonal Antibody AML-1 |
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| Catalog No: |
IMG-373 |
| Contents: |
100 ug in 200 ul of PBS containing 0.2% gelatin, 0.05% sodium azide. Sodium azide is highly toxic |
| Isotype: |
Rabbit IgG |
| Clone: |
N/A |
| Purification: |
Protein G Chromatography |
| Species: |
Human, Mouse |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 2-5 ug/ml |
| Storage: |
It is supplied as Protein G purified IgG. Store at 4°C, for long term store at –20°C. |
Recommended
Positive Control: |
Ramos, Raw |
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Background
AML-1 is a DNA-binding protein which specifically interacts with a sequence belonging to the group of enhancer core motifs, TGT/cGGT. The AML1 gene on chromosome 21 is disrupted in the (8;21)(q22;q22) translocation associated with acute myelogenous leukemia and encodes a protein with a central 118-amino-acid domain with 69% homology to the Drosophila pair-rule gene, runt. AML-1 translocation is frequently observed in acute myeloid leukemia. It is a key transactivator of tissue-specific genes of the hematopoietic and bone lineages. |
Antigen
Rabbit anti-AML-1 polyclonal antibody was raised against a peptide corresponding to a sequence that is homologous in AML-1 and AML-1/ETO [amino acids 231 to 245 (AFNPQPQSQMQDTRQ) of human origin]. |
Genebank Info (Protein)
NP_005773 |
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Western blot analysis of AML-1 in (A) human Ramos and(B) mouse Raw cell lysate using IMG-373 at 5 ug/ml. |
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Reference
1. Davis JN, McGhee L, Meyers S. The ETO (MTG8) gene family. Gene. 2003 Jan 16;303:1-10.
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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