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Product : Antibody >> DNA Methylation & Rep.. >> Dnmt2
      
 

Monoclonal Antibody to Dnmt2 (DNA methyltransferase 2)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-374
Dnmt2Purified
0.1 mg
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Catalog No: IMG-374
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 0.1 mg antibody in 0.2 ml of PBS containing 0.2% gelatin and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Mouse IgG1, Kappa
Clone: 102B1259.2
Purification: Protein G Chromatography
Species: Human, Mouse
Host: Mouse
Application: Western blot analysis: use antibody at 1-3 ug/ml
Storage: Store Dnmt2 antibody at 4°C, stable for 6 months. For long-term storage, aliquot and store antibody at -20°C.
Recommended
Positive Control:
transfected cell lysate
 

Background
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. Dnmt2 contains all the sequence motifs diagnostic of DNA (cytosine-5)-methyltransferases but appears to lack the large N-terminal regulatory domain common to other eukaryotic methyltransferases.

Antigen
This antibody was raised against synthetic peptides corresponding to mouse Dnmt2.

Genebank Info (Protein): NP_788271
Gene ID: 1787
SwissProt: Q6ICS7
 
Dnmt2 Antibody

Western blot analysis of Dnmt2 in transfected cell lysate using Dnmt2 monoclonal antibody at 2 µg/ml.

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Reference
1. Yoder JA, Bestor TH.  A candidate mammalian DNA methyltransferase related to pmt1p of fission yeast.  Hum Mol Genet. 1998 Feb;7(2):279-84.
2. Okano, M., Xie, S., and Li, E. Dnmt2 is not required for de novo and maintenance methylation of viral DNA in embryonic stem cells. Nucleic Acids Res 1998 26, 2536-40.
3. Hermann A, Schmitt S and Jeltsch A (2003). "The human Dnmt2 has residual DNA-(cytosine-C5) methyltransferase activity." J. Biol. Chem. 278(34): 31717-31721.
4. Franchina M, Hooper J, Kay PH. Five novel alternatively spliced transcripts of DNA (cytosine-5) methyltransferase2 in human peripheral blood leukocytes. Int J of Biochem Cell. Biol 33: 1104-1115 (2001).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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