Monoclonal Antibody to Dnmt2 (DNA methyltransferase 2)

Background
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. Dnmt2 contains all the sequence motifs diagnostic of DNA (cytosine-5)-methyltransferases but appears to lack the large N-terminal regulatory domain common to other eukaryotic methyltransferases.

Antigen
This antibody was raised against synthetic peptides corresponding to mouse Dnmt2.

Reference
1. Yoder JA, Bestor TH.  A candidate mammalian DNA methyltransferase related to pmt1p of fission yeast.  Hum Mol Genet. 1998 Feb;7(2):279-84.
2. Okano, M., Xie, S., and Li, E. Dnmt2 is not required for de novo and maintenance methylation of viral DNA in embryonic stem cells. Nucleic Acids Res 1998 26, 2536-40.
3. Hermann A, Schmitt S and Jeltsch A (2003). "The human Dnmt2 has residual DNA-(cytosine-C5) methyltransferase activity." J. Biol. Chem. 278(34): 31717-31721.
4. Franchina M, Hooper J, Kay PH. Five novel alternatively spliced transcripts of DNA (cytosine-5) methyltransferase2 in human peripheral blood leukocytes. Int J of Biochem Cell. Biol 33: 1104-1115 (2001).