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Peptide-affinity Purified Polyclonal Antibody to BID |
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| Catalog No: |
IMG-3812 |
| Contents: |
0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Isotype: |
Goat Ig |
| Clone: |
N/A |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Human |
| Host: |
Goat |
| Application: |
Western blot analysis:0.1-0.3µg/ml
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| Storage: |
Store at -20°C. |
Recommended
Positive Control: |
A431 lysate |
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Background
Principal Names- BID; BH3 interacting domain death agonist ; HGNC:1050; MGC15319; MGC42355 ; BH3-interacting domain death agonist; Human BID coding sequence; apoptic death agonistOfficial Symbol: BIDGenBank Accession Number(s): NP_932070.1; NP_001187.1; |
Antigen
Peptide with sequence C-TYVRSLARNGMD, from the C Terminus of the protein sequence according to NP_932070.1; NP_001187.1; NP_932071.1. |
Application Notes
Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 26+24kDa bands observed in Human fibroblast A431 lysates (calculated MW of 26.8kDa according to NP_932070.1 and 22.0kDa according to NP_001187.1). Recommended concentration: 0. |
| Genebank Info (Protein): NP_001187 |
| Gene ID: 637 |
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Western blot analysis of A431 lysate (35µg protein in RIPA buffer) using IMG-3812 (0.1µg/ml). |
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Reference
1. Lund T, Stokke T, Olsen OE, Fodstad O.
Garlic arrests MDA-MB-435 cancer cells in mitosis, phosphorylates the proapoptotic BH3-only protein Bim(EL) and induces apoptosis.
Br J Cancer. 2005 Apr 12;
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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