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Polyclonal Antibody to APE (APE1) |
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| Catalog No: |
IMG-392 |
| Contents: |
100 ug antibody in 200 ul PBS containing 0.02% sodium azide. Sodium azide is highly toxic |
| Isotype: |
Rabbit IgG |
| Clone: |
N/A |
| Purification: |
Protein G Chromatography was used to purify the APE antibody |
| Species: |
Human |
| Host: |
Rabbit |
| Application: |
IHC-P: use antibody at 10 ug/ml
Western blot analysis: 2-5 ug/ml |
| Storage: |
Store APE antibody at 4°C for six months. For long term storage, store antibody at -20°C. |
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Background
Human apurinic (apyrimidinic) endonuclease/redox-factor 1 (APE, APE1, Ref-1) is involved in the repair of DNA damage as well as in the transcriptional regulation of genes. The repair function of the protein is located on its C-terminal region. Activation of transcription factors, which occurs via a redox-based mechanism, pertains to a approximately 6 kDa N-terminal fragment with Cys-65 being of essential importance. APE has been shown to stimulate the DNA binding activity of numerous transcription factors that are involved in cancer promotion and progression such as Fos, Jun, NF (B, PAX, HIF-1(, HLF and p53. APE is part of the cellular response to oxidative stress and protects cells from the genotoxic and cytotoxic effect of oxidizing agents. Thus, specific downmodulation of APE activity in tumors might be considered as a novel therapeutic approach in tumor therapy. |
Antigen
This antibody was developed against KLH-conjugated synthetic peptides corresponding to amino acids 36-52 and 216-232 of human APE. |
Genebank Info (Protein)
NP_001632 |
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Western blot analysis of APE in HeLa lysates using APE antibody at 2 ug/ml. |
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Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE) using APE antibody at 10 ug/ml. |
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Reference
1. Jiricny J. Nature 415(6872):593-4 (2002).
2. Demple B, Herma T, Chen DS. Proc Natl Acad Sci USA 88: 11450-11454 (1991).
3. Fritz G.. Int J Biochem Cell Biol 32 (9): 925-9 (2000).
4. Evans AR, Limp-Foster M, Kelley MR. Mutat Res 461(2):83-108 (2000).
5. Puglisi F, Barbone F, Tell G, Aprile G, Pertoldi B, Raiti C, et al. Oncol Rep 9(1):11-17 (2002).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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