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Datasheet
Monoclonal Antibody to Cleaved Retinoblastoma Protein (DRb-p70)
Cat.No IMG-395
Description Rb-cleaved (DRb-p70)
Format Purified
Unit 0.1 mg
Price(USD) 257.00
MSDS
Catalog No
:
IMG-395
Contents
:
100 ug of antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype
:
Mouse IgG1
Clone
:
172C1094
Purification
:
Protein G Chromatography
Species
:
Human, Mouse
Host
:
Mouse
Application
IHC: (see Liu et al, 2005 in Product Citation) Western blot analysis: 2 ug/ml Storage It is supplied as Protein G purified IgG fraction. Store at 4oC, stable for 6 months. For long-term storage, store at -20oC. Recommended Positive Control : camptothecin-treated HL60 (24 hr)
Background The retinoblastoma gene product (Rb) is a tumor suppressor protein and acts as cell cycle regulator (1). Recently, Rb has been shown to play a role in apoptosis. During apoptosis, Rb is subjected to cleavage by caspase-3 like protease. Caspase-3 cleaves Rb into 48 and 68kDa fragments (2-4). Cleavage of Rb would make it functionally inactive, causing release of E2F-1. The E2F-1 then may promote apoptosis (5).
Antigen
A synthetic peptide corresponding to a region of the caspase-3-induced cleavage site of human Rb was used as immunogen.
Application Notes
The antibody recognizes cleaved Rb (DRb-p70). The antibody does not recognize full-length Rb. In camptothecin treated HL60, an ~68 kDa band is observed.
DRb-p70 exists in vivo as an N-terminally truncated form of Rb (Liu et al.). DRb-p70 is thought to be the product of alternative translation and is expressed in primary myeloid cells in fetal liver, bone marrow and spleen.
DRb-p70 is also specifically expressed in tumor-associated macrophages (TAMs). TAMs are an important targets of cancer therapy. DRb-p70 is a marker of TAMs, and maybe useful for identifying tumors with macrophage infiltration (Liu et al, 2005).
Western blot analysis: HL60 cells were treated with camptothecin for 24 hours.
Immunohistochemistry of DRb-p70 using IMG-395 in sections of primary human breast tumors. Image courtesy of Dr. Macleod, The Ben May Institute for Cancer Research, The University of Chicago
Reference 1. Weinberg RA. Science 254: 1138-1146 (1991). 2. An B and Dou QP. Cancer Res. 56: 438-442 (1996). 3. Dou QP, An B, Antouku K, and Johnson DE. J. Cell. Biochem 64: 586-594 (1997). 4. Fattman CL, Delach SM, Dou QP, Johnson DE. Oncogene 20: 2918-2926 (2001). 5. Almasan A, Yin Y, Kelly RE, Lee EYHP, Bradley A, Li W, Bertino JR and Wahl GM. Proc Natl Acad Sci USA, 92: 5436-5440 (1995).
Product Citations 1. A novel form of pRb expressed during normal myelopoiesis and in tumour-associated macrophages. H. P. Liu, A. M. Thompson and K. F. Macleod. Cell Proliferation. Volume 38 Issue 1 Page 13- February 2005. doi:10.1111/j.1365-2184.2005.00326.x (WB, IHC on paraffin section)
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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