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Product : Antibody >> GTPase , Signal Transduction >> GEF5
      
 

Peptide-affinity Purified Polyclonal Antibody to GEF5
 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-3973
GEF5Purified, Peptide Affinity
0.1 mg
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Catalog No: IMG-3973
Contents: 0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Isotype: Goat Ig
Clone: N/A
Purification: Antigen Affinity Chromatography
Species: Human
Host: Goat
Application: ELISA: >1:16,000
Western blot analysis: 0.1-0.3µg/ml
Storage: Store at -20°C.
Recommended
Positive Control:		 HeLa lysate
 

Background
Principal Names- ARHGEF5; Rho guanine nucleotide exchange factor (GEF) 5; DKFZp686N1969; GEF5; P60; TIM; TIM1; guanine nucleotide regulatory protein TIM; oncogene TIM rho guanine nucleotide exchange factor 5; transforming immortalized mammary oncogene. Official Symbol- ARHGEF5. GenBank Accession Number(s)- NP_005426.2. GeneID- 7984 (human).

Antigen
Peptide with sequence C-DKEIDQNSQQEE, from the internal region of the protein sequence according to NP_005426.2.

Application Notes
Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 170kDa band observed in Human HeLa lysates (calculated MW of 177kDa according to NP_005426.2). Recommended concentration: 0.1-0.3µg/ml.

Genebank Info (Protein): NP_005426
Gene ID: 7984
SwissProt: Q12774
 
GEF5 Antibody

Western blot Analysis of GEF5 in HeLa lysate using IMG-3973 at 0.1µg/ml.
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Reference
1. Debily MA, Camarca A, Ciullo M, Mayer C, El Marhomy S, Ba I, Jalil A, Anzisi A, Guardiola J, Piatier-Tonneau D. Expression and molecular characterization of alternative transcripts of the ARHGEF5/TIM oncogene specific for human breast cancer. Hum Mol Genet. 2004 Feb 1;13(3):323-34. Epub 2003 Dec 8. PMID: 14662653
Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.

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All products are For Research Use Only, unless indicated otherwise. Not for use in diagnostic or therapeutic procedures.

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