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Product : Antibody >> Apoptosis , Cancer Antibodies , CD Markers , Cell Cycle , New Antibodies >> CD28
      
 

Polyclonal Antibody to CD28

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-4038
CD28Purified
0.1 mg
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Catalog No: IMG-4038
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 0.1 mg (1 mg/ml) in PBS containing 0.02% sodium azide.
Isotype: Rabbit IgG
Clone: N/A
Purification: Protein G purified
Species: Human
Host: Rabbit
Application: Western blot analysis: 1:500-1:1000
Storage: Maintain at +2-8C for 3 months or at -20C for longer periods. Stable for 1 year. Avoid repeated freeze-thaw cycles.
 

Background
Principal Names: CD28; Tp44; CD28 antigen; T-cell specific surface glycoprotein; Official Gene Symbol: CD28 Gene ID: 940 (Human) Gene Map Locus: 2q33 (Human) CD28, a 44KDa homodimer and T-cell membrane glycoprotein, is a member of the Ig super family. It binds B7 molecules on APCs and transmits vital co-stimulatory signals required for proper T-cell proliferation, survival, IL-2 production and Th2 development. CD28 plays a vital role in recruitment of signaling molecules to the TCR including PI3K, PKB/Akt, Src Kinase Lck, Tec Kinases, PTKs, Vav-1, IKKalpha, Grb2, SLP76, p21ras and PAK. CD28/B7 interactions activate various signaling pathways including PI3K/Akt signaling, PTK-signaling, NF-kappaB activation and Rho-GTPase signaling. CD28 co-stimulation and ligation with TCR/CD3 complex directly controls T-cell cycle progression by down-regulating CDK inhibitor p27Kip1. These interactions also up-regulate Bcl-XL expression and down-regulate FasL expression thus playing a vital role in T-cell survival. Blocking of CD28/B7 pathway results in immunosuppression with implications for treatment of autoimmune diseases, organ transplantation and graft vs. host disease.

Antigen
Peptide corresponding to amino acids 72-85 [SQQLQVYSKTGFN].

Application Notes
the Ig-V-like region of human CD28.

Genebank Info (Protein): NP_006130
Gene ID: 940

Genebank Info (Nucleotide): NP_006130

 
CD28 Antibody

Western blot analysis of huCD28 in CD4+ cells using IMG-4038 at 1:500 dilution

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Reference
1. Hutloff, A. et. al. Nature (1999) 397: 263-266
2. Greenfield et.al Crit Rev Immunol., 18(5):389-418 (1998)
3. Y Colette et.al Blood, 92 (4) 1350-1363 (Aug 15 1998)
4. Piccollela et.al The Journal of Immunology 170: 2895-2903 (2003)
5. Fontana et.al The Journal of Immunology 171: 2225-2232 (2003)
6. Andres et.al Nat Immunol., 5(4):435-42 (2004 Apr)
7. Riley et.al Blood 105(1): 13 21 (January 1, 2005)


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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