Datasheet

Polyclonal Antibody to Defensin-3 (bD-3) Cat.No IMG-4090 Description Defensin-3 (bD-3) Format Purified Unit 0.1 mg Price(USD) 225.00 MSDS Catalog No : IMG-4090 Contents : 0.1 mg (1 mg/ml) in PBS containing 0.02% sodium azide. Isotype : Rabbit IgG Clone : N/A Purification : Protein G purified Species : Human Host : Rabbit Application Western blot analysis: 1:500-1:1000 Storage Maintain at +2-8°C for 3 months or at -20°C for longer periods. Stable for 1 year. Avoid repeated freeze-thaw cycles. Background A novel, nonhemolytic antimicrobial peptide (human beta-defensin-3, hBD-3) was isolated from human lesional psoriatic scales and cloned from keratinocytes. hBD-3 demonstrated a salt-insensitive broad spectrum of potent antimicrobial activity against many potentially pathogenic microbes including multiresistant S. aureus and vancomycin-resistant Enterococcus faecium. Keratinocytes and airway epithelial cells are cellular sources of hBD-3. Tumor necrosis factor alpha and contact with bacteria were found to induce hBD-3 mRNA expression. hBD-3 therefore might be important in the innate epithelial defense of infections by various microorganisms seen in skin and lung, such as cystic fibrosis. Antigen Peptide corresponding to amino acids 23-33 [GIINTLQKYYC] of the 5-kDa human beta-defensin-3. Genebank Info (Protein) NP_061131   ffsdf Related Products 1. 20301 [Goat Anti-Rabbit HRP Conjugate] Reference 1. Harder J, Bartels J, Christophers E, and Schroder JM. J Biol Chem 2001 Feb 23;276(8):5707-13 Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.

Related Products

Related Product(s) of the Catalog # IMG-4090

Cat. No Description Format Species Appl. Qty Price(USD) 20301 Anti-Rabbit Ig HRP Conjugate HRP R ELISA, WB 0.1 mL 80.00

FAQ

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Protocols

Imgenex Protocols
1.	Histo-Array Protocols
The protocols described here can be used as a guideline for use of IMGENEX Histo-array slides. Modifications may be necessary depending on the target gene(s), antibodies, or nucleic acid probes.
2.	INSTA-Blot Protocol
Western Blot protocol for INSTA-Blot Ready-to-Use membrane
3.	Custom Polyclonal Antibody Production Protocol
Custom Polyclonal Antibody Production Protocol
4.	IMGENEX Western Blot Protocol
IMGENEX Western Blot protocol
5.	Intracellular Staining Flow Protocol
Catalog No. 10083K
6.	Western Blot Protocol for Goat Antibodies
Western Blot Protocol for Goat Antibodies
7.	Tissue Lysate Preparation Protocol
Imgenex Tissue Lysate Preparation Protocol
8.	Cell Lysate Preparation Protocol
Imgenex Cell Lysate Preparation Protocol
9.	DNA Isolation
DNA Isolation
10.	IHC Protocol for Antibody Detection in Formalin fixed Tissue
IHC Protocol for Antibody Detection in Formalin fixed Paraffin Embedded Tissues
11.	Cell Surface Staining Flow Protocol
Catalog No. 10084K
12.	Immunohistochemistry Protocol for IMA Microarray Slides
The protocols described here can be used as a guideline for use of IMGENEX Histo-array slides. Modifications may be necessary depending on the target gene(s), antibodies, or nucleic acid probes.

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