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Polyclonal Antibody to CIDE-3 |
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| Catalog No: |
IMG-4158 |
| Contents: |
0.1 mg (1 mg/ml) in PBS containing 0.02% sodium azide. |
| Isotype: |
Rabbit IgG |
| Clone: |
N/A |
| Purification: |
Protein G purified |
| Species: |
Human |
| Predicted React: |
Mouse |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 1:500-1:1000
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| Storage: |
Maintain at +2-8°C for 3 months or at -20°C for longer periods. Stable for 1 year. Avoid repeated freeze-thaw cycles. |
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Background
DNA fragmentation is one of the critical steps in apoptosis, which is induced by DNA fragmentation factor (DFF). DFF is composed of two subunits, a 40 kDa caspase-activated nuclease (DFF40) and a 45 kDa inhibitor (DFF45). Recently a novel family of cell-death-inducing DFF45-like effectors (CIDEs) has been identified. Human CIDE-3 is a novel member of CIDEs. There are 2 transcripts, CIDE-3 and CIDE-3alpha, were present in HepG2 and A375 cells. Consistent with its chromosome localization at 3p25, a region associated with high frequency loss of heterozygosity in many tumours, CIDE-3 may play an important role in prevention of tumorigenesis. |
Antigen
Peptide corresponding to amino acids 10-25 [LLYPKSLSRHVSVRTS] of the 27 kDa human CIDE-3 protein. |
Application Notes
The mouse sequence is 93% positive, 80% identical to hCIDE-3, so this antibody may react with the mouse homolog FSP27. |
| Genebank Info (Protein): NP_071377 |
| Gene ID: 63924 |
| SwissProt: Q96AQ7 |
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Western blot analysis of CIDE-3 using IMG-4158 at 1:1000 dilution on 30ug HepG2 cell lysate. |
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Reference
1. Liang, L. et al. Biochem. J. 370 (Pt 1), 195-203 (2003)
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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