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Monoclonal Antibody against human TLR2 (Toll-like receptor 2)/CD282 |
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| Catalog No: |
IMG-416A |
| Contents: |
100 µg in 200 µl PBS containing, 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Mouse IgG2a |
| Clone: |
TL2.1 |
| Purification: |
Protein G Chromatography |
| Species: |
Dog, Human |
| Host: |
Mouse |
| Application: |
Flow (Cell Surface): 1-2 ug/10^6 cells
Flow (Intracellular): 1-2 ug/10^6 cells
IF/ICC: please see Mempel et al. (2003) for details
IHC (paraffin): please see Faure et al. (2001) for details
IP: 2-5 ug
Western blot analysis: please see Faure et al. (2001) for details |
| Storage: |
Store at 4°C, stable for 6 months. For long-term storage, store at -20°C. |
Recommended
Positive Control: |
PBMCs, Ramos |
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Background
The Toll-like receptors (TLRs) in mammals comprise a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and an IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). TLR2 is differentially expressed in human cells. TLR2 is expressed in tonsils, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells did not express TLR2. The expression of TLR2 in different cell types is regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes (4). |
Antigen
This antibody was raised by immunizing mice with CHO cells transfected with human TLR2 cDNA (Flo et al, 2000). The hybridoma supernatants were selected by flow cytometry. |
Application Notes
For a publicaton using this antibody on dog, please see Bazzocchi et al, 2005. |
Genebank Info (Protein)
NP_003255 |
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Cell surface flow analysis of TLR2 in 10^6 PBMCs using 1 ug of IMG-416A. Shaded histogram represents cells without antibody; green represents isotype control; red represents anti-TLR2 antibody. IMGENEX's goat anti-mouse IgG PE secondary (IMGENEX, 20103) was used in this test. |
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Reference
1. Chuang TH and Ulevitch RJ. Eur Cytokine Netw 11(3): 372-378 (2000).
2. Muzio M, Natoli G, Saccani S, Levrero M, and Mantovani A. J. Exp. Med. 187: 2097-2101 (1998).
3. Medzhitov R and Janeway CA. Cell 91: 295-298 (1997).
4. Chuang TH and Ulevitch RJ. Biochim. Biophys. Acta 1518 (1-2): 157-161 (2001).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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