|
|
|
| |
Polyclonal Antibody to TLR9/CD289 |
|
|
| |
|
|
| |
|
| Catalog No: |
IMG-431 |
| Contents: |
0.1mg in 0.2 ml PBS containing 0.05% BSA, 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Purification: |
Protein G Chromatography |
| Species: |
Human, Mouse |
| Host: |
Rabbit |
| Application: |
Flow (Cell Surface): see Patole et al (2006) for details.
Flow (Intracellular): 0.5-1 ug/10^6 cells
IF/ICC: see Merrel et al. (2006) for details
IHC (frozen): 10 ug/ml, see Patole et al (2006) for details.
IHC (paraffin): 5 ug/ml or see Tran et al. (2007)
Western blot analysis: 0.5-1 ug/ml |
| Storage: |
Store at 4ºC, stable for 6 months. For long-term storage, store at -20ºC. |
Recommended
Positive Control: |
mouse intestine |
|
 |
|
|
|
|
|
|
Background
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). By using a BLAST search, Hemmi et al., 2000 (4) have identified and subsequently isolated a cDNA coding for TLR9. Gene knockout experiments suggest that TLR9 acts as a receptor for unmethylated CpG dinucleotides in the bacterial DNA (4). Human and mouse TLR9 share an overall amino acid identity of 75.5%. TLR9 is highly expressed in spleen. |
Antigen
This antibody was developed against a KLH-conjugated synthetic peptide corresponding to part of the extracellular domain of mouse TLR9, between amino acids 200-300. |
Application Notes
1. In mouse intestine, a 113 kDa band is observed by western blot.
2. TLR9 was upregulated in LnCap prostate carcinoma cells following Estradiol stimulation (Ilvesaro et al, 2007).
3. For FC, use RAW or thioglycolate-elicited mouse macrophage cells. |
Genebank Info (Protein)
NP_059138 |
| |
|
Western blot analysis of mouse TLR9 in cell lysates from mouse intestine using IMG-431 at a dilution of 0.5 µg/ml. The observed size of TLR9 is different in human and mouse samples. IMGENEX’s goat anti-rabbit Ig HRP secondary antibody (IMGENEX, 20301) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test. |
|
|
|
|
Intracellular flow analysis of TLR9 in 10^6 human PBMCs (lymphocyte-gated) using 0.5 ug of antibody. Shaded histogram represents cells without antibody, green represents isotype control (Imgenex, 20304), red represents IMG-431. Imgenex's goat anti-rabbit PE secondary antibody (20303) was used. |
|
|
|
|
Immunohistochemical analysis of TLR9 in paraffin-embedded formalin-fixed human breast tissue using an isotype control (left) and IMG-431 (right) at 5 ug/ml. |
|
|
|
|
|
| - |
 |
|
Reference
1. Muzio M, Natoli G, Saccani S, Levrero M, and Mantovani A. J. exp. Med. 187: 2097-2101 (1998).
2. Medzhitov R and Janeway CA. Cell 91: 295-298 (1997).
3. Chuang TH and Ulevitch RJ. Biochim. Biophys. Acta 1518 (1-2):157-161 (2001)
4. Takeuchi O, Kawai T, Sanjo H, Copeland NG, Gilbert DJ, Jenkins NA, Takeda K, and Akira S. Gene 231: 59-65 (1999).
5. Hemmi H, Takeuchi O, Kawai T, Kaisho T, Sato S, Sanjo H, Matsumoto M, Hoshino K, Wagner H, Takeda K, and Shizo A. Nature 408: 740-745 (2000).
|
|
|
|
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
|
|
|
|
|
