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Product : Antibody >> Apoptosis , Cancer Antibodies , Cytokine & Chemokine , IHCProTM , Immunohistochemistry >> STAT6
      
 

Polyclonal Antibody to STAT6

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-462
STAT6Purified
0.2 mL
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Catalog No: IMG-462
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: STAT6 antibody in 200 ul PBS containing 0.2% gelatin and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit IgG
Purification: Protein G Chromatography was used to purify this STAT6 antibody
Species: Human
Predicted React: Mouse, Rat
Host: Rabbit
Application: IHC (p): 1:50
Western blot analysis: use STAT6 antibody at 1:500-1:1000
Storage: Store STAT6 antibody at 4oC, stable for 6 months. For long-term storage, store antibody at -20oC.
 

Background
STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. Stat 6 is rapidly activated in response to IL-4, however the fate of the activated form is not clear.

Antigen
This antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acids 806-826 (ILPRQQYFKQNCSQHESSP) of human STAT6. This sequence is 80% homologous in mouse and 85% homologous in rat.

Application Notes
The STAT6 amino acid sequence used as immunogen for this antibody is 80% homologous in mouse and 85% in rat.

Genebank Info (Protein): NP_003144
Gene ID: 6778
 
STAT6 Antibody

Western blot analysis of STAT6 in K562 cell lysate using STAT6 antibody at 1:1000 dilution.

STAT6

Formalin-fixed, paraffin-embedded thymus tissue (medulla) stained STAT6 antibody (1:50).

Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

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Reference
1. Leonard WJ, et al. Annu Rev Immunol 16:293-322 (1998).
2. Schindler C, et al. Annu Rev Biochem 64:621-51 (1995).
3. Bluyssen HA and Levy DE. J Biol Chem 272: 4600-4605 (1997).
4. Darnell JE Jr. Science 277:1630-1635 (1997).
5. Quelle FW, et al. Mol Cell Biol 15(6): 3336-43. (1995).
6. Ryan P., et al. J. Biol. Chem. published July 16, 2002
7. Akinori Kanai, Kenta Suzuki, Kousuke Tanimoto, Junko Mizushima-Sugano, Yutaka Suzuki, Sumio Sugano. Characterization of STAT6 Target Genes in Human B Cells and Lung Epithelial Cells. DNA Res. 2011 October; 18(5): 379–392.


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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