Polyclonal Antibody to DUT-N (Deoxyuridine triphosphate nucleotidohydrolase)

Background
Deoxyuridine triphosphate nucleotidohydrolase (dUTPase) is a ubiquitous enzyme that functions in the hydrolysis of dUTP to dUMP and pyrophosphate. Canman and co-workers have demonstrated that, in certain human tumor cell lines, increased levels of dUTPase are responsible for an increase in resistance to the cancer chemotherapeutic agent fluorodeoxyuridine (FUdR), a thymidine synthase inhibitor. Two distinct forms of dUTPase exist in humans. Cellular fractionation experiments suggest that the more abundant, lower mass form of dUTPase (DUT-N) localizes in the nucleus, while the higher mass form (DUT-M) is associated with the mitochondria.

Antigen
This antibody was developed against a mixture of KLH-conjugated synthetic peptides corresponding to amino acids 25-44 and 113-130 of human DUT-N.

Reference
1. McIntosh, E. M., Ager, D. D., Gadsden, M. H., and Haynes, R. H. (1992) Proc. Natl. Acad. Sci. U. S. A. 89, 8020-8024Fritz G.. Int J Biochem Cell Biol 32 (9): 925-9 (2000).
2. Ladner RD, McNulty DE, Carr SA, Roberts GD, Caradonna SJ. J. Biol Chem. 271 (13) 7745-7751 (1996).