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Product : Antibody >> DNA Methylation & Rep.. >> DUT-N
      
 

Polyclonal Antibody to DUT-N (Deoxyuridine triphosphate nucleotidohydrolase)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-465
DUT-NPurified
0.2 mL
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Catalog No: IMG-465
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: Antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit Ig
Clone: N/A
Purification: Protein G Chromatography
Species: Human
Host: Rabbit
Application: Western blot analysis: 1:1000-1:2000
Storage: Store at 4C for six months. For long term storage, aliquot and store at -20C.
Recommended
Positive Control:
HeLa
 

Background
Deoxyuridine triphosphate nucleotidohydrolase (dUTPase) is a ubiquitous enzyme that functions in the hydrolysis of dUTP to dUMP and pyrophosphate. Canman and co-workers have demonstrated that, in certain human tumor cell lines, increased levels of dUTPase are responsible for an increase in resistance to the cancer chemotherapeutic agent fluorodeoxyuridine (FUdR), a thymidine synthase inhibitor. Two distinct forms of dUTPase exist in humans. Cellular fractionation experiments suggest that the more abundant, lower mass form of dUTPase (DUT-N) localizes in the nucleus, while the higher mass form (DUT-M) is associated with the mitochondria.

Antigen
This antibody was developed against a mixture of KLH-conjugated synthetic peptides corresponding to amino acids 25-44 and 113-130 of human DUT-N.

Genebank Info (Protein): NP_001939
Gene ID: 1854
SwissProt: P33316
 
DUT-N Antibody

Western blot detection of DUT-N in HeLa cell lysate using IMG-465 at a dilution of 1:1000.

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Reference
1. McIntosh, E. M., Ager, D. D., Gadsden, M. H., and Haynes, R. H. (1992) Proc. Natl. Acad. Sci. U. S. A. 89, 8020-8024Fritz G.. Int J Biochem Cell Biol 32 (9): 925-9 (2000).
2. Ladner RD, McNulty DE, Carr SA, Roberts GD, Caradonna SJ. J. Biol Chem. 271 (13) 7745-7751 (1996).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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