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Product : Antibody >> DNA Methylation & Rep.. >> CGBP
      
 

Polyclonal Antibody to CGBP (CpG binding protein)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-477
CGBPPurified
0.2 mL
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Catalog No: IMG-477
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ug antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit Ig
Clone: N/A
Purification: Protein G Chromatography
Species: Human
Host: Rabbit
Application: Western blot analysis: 1:1000
Storage: Store at 4C, stable at 4C for 6 months. For long-term storage, store at -20C.
Recommended
Positive Control:
human spleen
 

Background
Human CpG binding protein (hCGBP) contains three cysteine-rich domains, two of which exhibit homology to the plant homeodomain finger domain and the third cysteine-rich domain conforms to the CXXC motif identified in DNA methyltransferase, human trithorax, and methyl-CpG binding domain protein 1. A fragment of hCGBP that contains the CXXC domain binds to an oligonucleotide probe containing a single CpG site, and this complex is disrupted by distinct oligonucleotide competitors that also contain a CpG motif(s). CBGP plays an important part in embryonic viability and peri-implantation development. Native hCGBP is detected as an 88-kDa protein by Western analysis and is ubiquitously expressed. CGBP trans-activates promoters that contain CpG motifs but not promoters in which the CpG is ablated suggesting that hCGBP is a transcriptional activator that recognizes unmethylated CpG dinucleotides.

Antigen
This polyclonal antibody was raised against a mixture of synthetic peptides containing 73-90 and 641-656 amino acids residues of human CGBP.

Genebank Info (Protein): NP_001095124
Gene ID: 30827
SwissProt: Q9P0U4
 
CGBP Antibody

Western blot analysis of CGBP using IMG-477 at 1:1,000 dilution against 15 ug/lane of human spleen lysate. Immunoreactivity was determined using SuperSignal system (Pierce) and by exposing to the film for 15 minutes.

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Reference
1. Voo KS, Carlone DL, Jacobsen BM, Flodin A, and Skalnik DG. Mol Cell Biol, 20 (6): 2108-2121 (2000).
2. Carlone DL, Skalnik DG. Mol Cell Biol 21(22):7601-7606 (2001).
3. Lee JH, Skalnik DG. J Biol Chem 2002 Aug 27; [epub ahead of print]


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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