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Polyclonal Antibody to CGBP (CpG binding protein) |
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| Catalog No: |
IMG-477 |
| Contents: |
Polyclonal antibody in 200 ul PBS containing 0.02% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit Ig |
| Clone: |
N/A |
| Purification: |
Protein G Chromatography |
| Species: |
Human |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 1:1000 |
| Storage: |
Store at 4șC, stable at 4șC for 6 months. For long-term storage, store at -20șC. |
Recommended
Positive Control: |
human spleen |
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Background
Human CpG binding protein (hCGBP) contains three cysteine-rich domains, two of which exhibit homology to the plant homeodomain finger domain and the third cysteine-rich domain conforms to the CXXC motif identified in DNA methyltransferase, human trithorax, and methyl-CpG binding domain protein 1. A fragment of hCGBP that contains the CXXC domain binds to an oligonucleotide probe containing a single CpG site, and this complex is disrupted by distinct oligonucleotide competitors that also contain a CpG motif(s). CBGP plays an important part in embryonic viability and peri-implantation development. Native hCGBP is detected as an 88-kDa protein by Western analysis and is ubiquitously expressed. CGBP trans-activates promoters that contain CpG motifs but not promoters in which the CpG is ablated suggesting that hCGBP is a transcriptional activator that recognizes unmethylated CpG dinucleotides. |
Antigen
This polyclonal antibody was raised against a mixture of synthetic peptides containing 73-90 and 641-656 amino acids residues of human CGBP. |
Genebank Info (Protein)
NP_001095124 |
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Western blot analysis of CGBP using IMG-477 at 1:1,000 dilution against 15 ug/lane of human spleen lysate. Immunoreactivity was determined using SuperSignal system (Pierce) and by exposing to the film for 15 minutes. |
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Reference
1. Voo KS, Carlone DL, Jacobsen BM, Flodin A, and Skalnik DG. Mol Cell Biol, 20 (6): 2108-2121 (2000).
2. Carlone DL, Skalnik DG. Mol Cell Biol 21(22):7601-7606 (2001).
3. Lee JH, Skalnik DG. J Biol Chem 2002 Aug 27; [epub ahead of print]
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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