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Monoclonal Antibody to Punctin |
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| Catalog No: |
IMG-485 |
| Contents: |
Antibody in 200 ul PBS containing 0.02% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Mouse IgM, Kappa |
| Clone: |
30D445.1 |
| Purification: |
Ascites |
| Species: |
Human, Mouse, Rat |
| Host: |
Mouse |
| Application: |
Western blot analysis: 1:250-1:500 |
| Storage: |
Store at 4°C. For long-term storage, store at -20°C. |
Recommended
Positive Control: |
human heart |
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Background
Punctin (ADAMTSL-1) is a secreted molecule, expressed in adult skeletal muscle, resembling members of the ADAMTS family of proteases, containing four thrombospondin type I repeats. Human punctin cleaves aggrecan and is thereby classified as an “aggrecanase.” Like the ADAMTS proteases, each domain in punctin has an even number of cysteine residues suggesting that each domain may have internal disulfide bonds and that punctin consists of a series of independently folded and disulfide-bonded domains. |
Antigen
This monoclonal antibody was raised against a synthetic peptide containing amino acid residues 226-240 (YLETKTLQGTKGENS) of human punctin, GenBank no. gi|21327691|ref|NP_443098.2| The immunogen sequence is 100% conserved in rat and mouse. |
Application Notes
Punctin may be expressed as various isoforms with varying molecular wieght. The punctin antibody recognizes isoforms which contain the sequence used for immunogen ((YLETKTLQGTKGENS). |
Genebank Info (Protein)
NP_001035362 |
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Western Blot analysis of punctin using IMG-485 at a 1:250 dilution against human heart. |
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Reference
1. Hirohata, S. Et al. J Biol Chem. 2002 Apr 5;277(14): 12182-9.
2. Rodriguez-Manzaneque JC. Et al. Biochem Biophys Res Commun. 2002 Apr 26;293(1): 501-8.
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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