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Polyclonal Antibody to AKT1 |
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| Catalog No: |
IMG-487 |
| Contents: |
Antibody in 200 ul PBS containing 0.02% sodium azide and 0.1 mg/ml BSA. Sodium azide is highly toxic. |
| Isotype: |
Rabbit Ig |
| Clone: |
N/A |
| Purification: |
Protein G Chromatography |
| Species: |
Human |
| Predicted React: |
Chicken, Frog, Mouse |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 1:500-1:1000 |
| Storage: |
It is supplied as Protein G purified Ig fraction. Store at 4oC, stable for 6 months. For long-term storage, store at -20oC. |
Recommended
Positive Control: |
Jurkat |
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Background
AKT, protein kinase B (PKB), is a serine/threonine kinase which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activat phosphtidylinositol 3-kinase (PI 3-kinase). The major activity of AKT/PKB is to mediate cell survival. AKT/PKB is also believed to be a critical factor in the genesis of cancer as the tumor suppressor PTEN was found to antagonise PI-3 kinase and AKT/PKB kinase activity. AKT/PKB phosphorylation is critical for it activity. The major phosphorylation sites required for AKT activation has been identified as threoinine 308 and serine 473. Serine 473 is phosphorylated by MAPKAP kinase 2. |
Antigen
This polyclonal antibody was developed against a synthetic peptide corresponding to amino acid residues 464-477 of human AKT1. This peptide sequence is identical in human, mouse, chicken and frog. |
Genebank Info (Protein)
NP_001014432 |
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Western blot analysis of AKT1 using IMG-487 at 1:1000 dilution against 15 ug/lane of Jurkat lysate. |
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Reference
1. Alessi DR., et al. EMBO J. 15: 6541-6551 (1996).
2. Ferring P., et al. Oncogene 18: 6129-6134 (1999).
3. Kandel ES, et al. Exp. Cell Res. 253: 210-219 (1999).
4. Cantley LC, et al. Proc Natl Acad Sci, USA, 96: 420-4245 (1999).
5. Franke TF, et al. Cell 88: 435-437 (1997).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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