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Respiratory Syncytial Virus |
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| Catalog No: |
IMG-4994 |
| Contents: |
0.1mg (1 mg/ml) in PBS containing 0.02% sodium azide. |
| Isotype: |
Rabbit IgG |
| Clone: |
N/A |
| Purification: |
Protein G Affinity Chromatography |
| Species: |
Viral |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 1:500 to 1:1,000 |
| Storage: |
Maintain at +2-8°C for 3 months or at -20°C for longer periods. Stable for 1 year. Avoid repeated freeze-thaw cycles. |
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Background
RSV is a negative sense RNA paramyxovirus in which the major virus neutralization antigens are the glycoprotein (G) and the fusion (F) protein that mediate attachment and fusion to the host cell during virus infection, respectively. Other RSV proteins include the nucleocapsid protein (N) and the M2 protein. The two major subgroups of RSV are A and B with the A strain being more virulent and responsible for the majority of severe RSV infections.
Respiratory syncytial virus (RSV) infection is responsible for a large proportion of viral respiratory infections, and significant morbidity is associated with RSV infections. To date, few RSV vaccine candidates have shown promise with no human vaccine yet been proven safe and efficacious. |
Antigen
H1N1 virions were used as immunogen. |
Application Notes
Whole RSV virions (subgroup A-Long strain) were used as an immunogen. Detects G, F, N and M2 proteins of RSV.Western blot – Use at 1:500 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application. |
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Western blot analysis of Respiratory Syncytial Virus. 3 ug of RSV virions (subgroup A-Long strain) were loaded on SDS-PAGE, transferred to a PVDF membrane and detected with rabbit anti-RSV (Long strain) Polyclonal Antibody at a 1:1,000 dilution. Goat anti-rabbit secondary antibody was used at 1:10,000 dilution. The antibody detects the following RSV proteins: Glycoprotein (G) ~90 kDa, Fusion (F) protein) ~55 kDa, and nucleocapsid (N) protein) ~46 kDa. Overexposure of the blot can result in detection of M2 protein (~22 kDA-not shown). |
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Reference
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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