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Polyclonal Antibody to TLR1 |
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| Catalog No: |
IMG-5012 |
| Contents: |
0.1 mg antibody in 0.2 ml PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Purification: |
Protein G Chromatography |
| Species: |
Human, Mouse, Rat |
| Host: |
Rabbit |
| Application: |
Flow (Cell Surface): 0.5-1 ug/10^6 cells, or see Wong et al, 2007
Flow (Intracellular): 0.5-1 ug/10^6 cells
IHC (p): 10 ug/ml
Western blot analysis: 1-3 ug/ml |
| Storage: |
Store TLR1 antibody at 4°C, stable for 6 months. For long-term storage, store antibody at -20°C. |
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Background
The Toll-like receptor (TLR) family in mammals are comprised of a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and an IL-1 receptor motif in the cytoplasmic domain. The TLRs could constitute an important and unrecognized component of innate immunity in humans. In mice, TLR1 interacts with TLR2 and coexpression of TLR1 and TLR2 enhance the NF-kappaB activation in response to a synthetic lipopeptide. They recognize the lipid configuration of the native mycobacterial lipoprotein as well as several triacylated lipopeptides. |
Antigen
This antibody was developed against a KLH-conjugated synthetic peptide corresponding to a sequence within amino acids 400-450 of human TLR1. |
Genebank Info (Protein)
NP_003254 |
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Western blot analysis of TLR1 in Ramos (A), Raw (B), and (C) TLR1 transfected cell lysate (IMGENEX, 40213) using IMG-5012 at 2 ug/ml. Imgenex's goat anti-rabbit HRP secondary antibody (IMGENEX, 20301) was used. |
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Cell surface flow cytometric analysis of TLR1 in human PBMCs (monocyte-gated) using IMG-5012 at 0.5 ug/10^6 cells. Shaded histogram represents cells without antibody, green represents isotype control (IMGENEX, 20304), red represents TLR1 antibody. IMGENEX's goat anti-rabbit PE secondary antibody (IMGENEX, 20303) was used. |
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IHC analysis of human small intestine using IMG-5012 at 10 µg/ml. |
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Reference
1. Takeuchi O, et al. J Immunol 169(1):10-14 (2002)
2. Wyllie, D.H., et al. J Immunol. 165: 7125 (2000).
3. Visintin, A., et al. J. Immunol. 166: 249 (2001).
4. Liu S, et al. Infect Immun 70(7):3433-42 (2002)
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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