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Product : Antibody >> Apoptosis , Neurobiology , New Antibodies , Nuclear & Cytoplasmic.. , Phosphospecific Antibodie.. >> Caveolin-2 (Ser23)
      
 

Affinity Purified Polyclonal Antibody to Caveolin-2 (Phospho-Ser23)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-5013
Caveolin-2 (Ser23)Purified, Peptide Affinity
0.05 mg
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Catalog No: IMG-5013
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 50 ug of purified IgG (0.5 mg/ml) with 50% glycerol, 0.01% thimerosal and 1.0 mg/ml BSA.
Isotype: Rabbit IgG
Clone: N/A
Purification: Protein G Chromatography
Species: Human
Host: Rabbit
Application: Western blot analysis: 0.1-0.5 ug/ml
Storage: Store at -20°C. Avoid multiple freeze/thaw cycles.
 

Background
Caveolae are specialized plasma membrane associated organelles that participate in numerous cellular functions including endocytosis, potocytosis, transcytosis, signal transduction and cholesterol trafficking. The major proteins that form caveolae are the caveolins of which there are three major isoforms. Caveolin-2 has been shown to positively regulate caveolin-1-dependent caveolae biogenesis; this phenomenon is contingent on the phosphorylation of caveolin-2 on serine 23 and 36.

Antigen
Rabbits were immunized with a synthetic peptide based on amino acid residues 18-31 of caveolin-2 phosphorylated on Ser23. Antibodies were purified by peptide affinity chromatography.

Application Notes
An ~20 kDa band is observed.

Genebank Info (Protein): NP_058596
Gene ID: 12390
SwissProt: Q9WVC3
 
Caveolin-2 (Ser23) Antibody

Lysates from LNCaP cells expressing nonphosphorylated and phosphorylated caveolin-2 were left untreated (-) or treated with (+) calf intestinal phosphatase (CIP).  The phosphospecific caveolin-2 (Ser23) (Cat No.IMG-5013) recognized the untreated, but not the CIP-treated lysate.

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Reference
1. Sowa, G., M. Pypaert, D. Fulton, and W.C. Sessa. 2003.  The phosphorylation of caveolin-2 and serines 23 and 36 modulates caveolin-1-dependent caveolae formation. PNAS 11:6511-6516. (Western blot)


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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