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Peptide-affinity Purified Caveolin-2 Polyclonal Antibody |
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| Catalog No: |
IMG-5015 |
| Contents: |
50 ug of purified IgG (0.5 mg/ml) with 50% glycerol, 0.01% thimerosal and 1.0 mg/ml BSA. |
| Isotype: |
Rabbit IgG |
| Clone: |
N/A |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Human |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 0.2-1 ug/ml |
| Storage: |
Store at -20°C. Avoid multiple freeze/thaw cycles. |
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Background
Caveolae are specialized plasma membrane associated organelles that participate in numerous cellular functions including endocytosis, potocytosis, transcytosis, signal transduction and cholesterol trafficking. The major proteins that form caveolae are the caveolins of which there are three major isoforms. Caveolin-2 has been shown to positively regulate caveolin-1-dependent caveolae biogenesis; this phenomenon is contingent on the phosphorylation of caveolin-2 on serine 23 and 36. |
Antigen
Rabbits were immunized with a synthetic peptide based on amino acid residues 18-31 of caveolin-2 phosphorylated on Ser-23. Antibodies were purified by peptide affinity chromatography. |
Application Notes
An ~20 kDa band is observed. |
Genebank Info (Protein)
NP_058596 |
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Lysates from LNCaP cells expressing nonphosphorylated and phosphorylated caveolin-2 were left untreated (-) or treated with (+) calf intestinal phosphatase (CIP). The caveolin-2 antibody (Cat No. IMG-5015) recognized both the untreated and CIP-treated lysates. |
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Reference
1. Sowa, G., M. Pypaert, D. Fulton, and W.C. Sessa. 2003. The phosphorylation of caveolin-2 and serines 23 and 36 modulates caveolin-1-dependent caveolae formation. PNAS 11:6511-6516. (Western blot)
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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