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Polyclonal Antibody to IkB alpha |
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| Catalog No: |
IMG-5016 |
| Contents: |
100 ug antibody in 200 ul PBS containing 0.2% gelatin and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Clone: |
N/A |
| Purification: |
Protein G Chromatography was used to purify this IkB alpha antibody |
| Species: |
Human |
| Predicted React: |
Mouse, Rat |
| Host: |
Rabbit |
| Application: |
IHC (p): use antibody at 10 ug/ml
Western blot analysis: 1-3 ug/ml |
| Storage: |
Store IkB alpha antibody at 4oC, stable for 6 months. For long-term storage, store antibody at -20oC. |
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Background
NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkB alpha is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first genes induced following NF-kB activation is IkB alpha. |
Antigen
A synthetic peptide corresponding to amino acids 34-48 of human IkB alpha was used as the immunogen for this antibody. It will cross-react with mouse and rat IkB alpha, based on BLAST. |
Application Notes
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| Genebank Info (Protein): NP_065390 |
| Gene ID: 4792 |
| SwissProt: P25963 |
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Western blot analysis of IkB alpha in 20 ug of human Jurkat lysate using IkB alpha antibody at 2 ug/ml. |
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IHC analysis of human breast using IkB alpha antibody at 10 µg/ml. |
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Reference
1. Verma, I.M., Genes. Dev. 9: 2723-2735, 1995.
2. Verma, I. And Stevenson, J.K. Proc. Nat. Acad. Sci USA 94: 11758 (1997).
3. DiDonato, J.A., et al. Nature 388: 548- (1997).
4. Regnier, C.H., et al. Cell 90, 373 (1997).
5. Mercurio, F., et al. Science 278: 860 (1997).
6. Yamaoka, S., et al. Cell 93: 1231-1240 (1998).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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